Repair of extensive ionizing-radiation DNA damage at 95 degrees C in the hyperthermophilic archaeon Pyrococcus furiosus
Repair of extensive ionizing-radiation DNA damage at 95 degrees C in the hyperthermophilic archaeon Pyrococcus furiosus
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DOI：
10.1128/jb.179.14.4643-4645.1997
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发表时间：
1997-07-01
影响因子：
3.2
通讯作者：
Robb, FT
中科院分区：
文献类型：
--
作者：
DiRuggiero, J;Santangelo, N;Robb, FT
我们通过测量在高水平Co-60 γ辐照下的存活情况，研究了嗜热性巨热球菌修复DNA的能力。在2500 Gy剂量下，P. furiosus 2-Mb染色体片段化，片段长度从500kb到30kb不等，并在95℃孵育后完全恢复。我们认为重组修复可能是P. furiosus一种非常活跃的修复机制，它可能是嗜热菌在高温下存活的重要决定因素。
We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of Co-60 gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented: into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95 degrees C. We suggest that recombination repair could be an extremely active repair mechanism in P. furiosus and that it might be an important determinant of survival of hyperthermophiles at high temperatures.