Decisive role of pH in synthesis of high purity fluorescent BSA-Au 20 nanoclusters
Decisive role of pH in synthesis of high purity fluorescent BSA-Au 20 nanoclusters
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pH 在高纯度荧光 BSA-Au 20 纳米团簇合成中的决定性作用
DOI：
10.1016/j.saa.2020.118520
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Hong Guo-Lin
中科院分区：
文献类型：
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作者：
Zhuang Quan-Quan;He Shao-Bin;Huang Kai-Yuan;Peng Hua-Ping;Chen Chang-Mai;Deng Hao-Hua;Xia Xing-Hua;Chen Wei;Hong Guo-Lin
牛血清白蛋白（BSA）保护的荧光金纳米团簇（BSA-AuNCs）已被制备并应用于各个领域。然而，传统的合成方法在溶液中的BSA-AuNCs通常产生低的光致发光量子产率（PLQY）。在这项研究中，我们系统地研究了孵育时间，温度和pH值对BSA-AuNCs形成过程的影响，然后开发了一种新的策略来合成具有PLQY（26%）的BSA-AuNCs，远远超过现有的同行。在pH、温度和时间这三个重要因素中，pH对形成具有不同组成和荧光性质的BSA-AuNCs起着关键作用。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）结果表明，在pH值为10以及孵育温度和反应时间的正确组合下，可以制备高纯度的BSA-Au 20 NCs。所获得的BSA-Au 20 NCs的优点包括小尺寸、高PLQY、长寿命、高纯度以及容易修饰，使它们成为成像和传感应用中的发光探针的理想候选者。
Various types of bovine serum albumin (BSA)-protected fluorescent gold nanoclusters (BSA-AuNCs) have been fabricated and applied in various fields. However, the conventional synthesis methods for BSA-AuNCs usually yield a low photoluminescence quantum yield (PLQY) in solution. In this study, we systematically examined the influences of incubation time, temperature, and pH on the formation process of BSA-AuNCs and then developed a novel strategy to synthesize BSA-AuNCs with PLQY (26%), far exceeding that of existing counterparts. Of the three important factors, pH, temperature, and time, pH plays a key role in the formation of BSA-AuNCs with different compositions and fluorescence properties. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) results showed that BSA-Au20NCs with high purity can be produced at a pH value of 10 and the correct combination of incubation temperature and reaction time. The advantages of the obtained BSA-Au20NCs, including small size, high PLQY, long lifetime, high purity, as well as facile modification, make them ideal candidates for luminescent probes in imaging and sensing applications.