14-3-3 σ possibly plays a constitutive role in papillary carcinoma, but not in follicular tumor of the thyroid
14-3-3 σ possibly plays a constitutive role in papillary carcinoma, but not in follicular tumor of the thyroid
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DOI：
10.1016/s0304-3835(03)00282-9
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发表时间：
2003-10-28
期刊：
影响因子：
9.7
通讯作者：
Miyauchi, A
中科院分区：
文献类型：
--
作者：
Ito, Y;Miyoshi, E;Miyauchi, A
14-3-3 σ是细胞周期的负调节因子，并有助于G2停滞。到目前为止，由于CpG岛的高甲基化，其表达的缺乏已在一些癌中报道。在这项研究中，我们研究了14-3-3 σ在甲状腺肿瘤中的表达，通过免疫组织化学和Western印迹分析。正常卵泡不表达14-3-3 σ。在82例乳头状癌中，14-3-3 σ均表达，且在进展期和低分化型中表达不降低，甚至增强。此外，23例间变性癌中有21例表达14-3-3或，其表达水平有高于乳头状癌的趋势。另一方面，34例滤泡性癌或29例滤泡性腺瘤均不表达14-3-3 sigma。这些结果表明，14-3-3 sigma在乳头状癌中起组成性作用，而不是作为细胞周期调节剂，而不是滤泡性肿瘤的发生和发展所必需的。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
14-3-3 sigma is a negative regulator of the cell cycle and contributes to G2 arrest. Thus far, the lack of its expression due to hypermethylation of the CpG islands has been reported in some carcinomas. In this study, we investigated the expression of 14-3-3 sigma in thyroid neoplasms by means of immunohistochemistry as well as Western blot analysis. Normal follicules did not express 14-3-3 sigma. In 82 papillary carcinomas, all the cases expressed 14-3-3 sigma and its expression was not reduced but even enhanced in the advanced stage and in poorly differentiated types. Furthermore, 21 of the 23 anaplastic carcinomas expressed 14-3-3 or and its expression level tended to be higher than in papillary carcinoma. On the other hand, none of the 34 follicular carcinomas or 29 follicular adenomas expressed 14-3-3 sigma. These results suggest that 14-3-3 sigma plays a constitutive role in papillary carcinoma rather than acting as a cell cycle regulator, whereas it is not required for the occurrence and development of follicular tumor. (C) 2003 Elsevier Ireland Ltd. All rights reserved.