Amplification and overexpression of TGIF2, a novel homeobox gene of the TALE superclass, in ovarian cancer cell lines
Amplification and overexpression of TGIF2, a novel homeobox gene of the TALE superclass, in ovarian cancer cell lines
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DOI：
10.1006/bbrc.2000.3449
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发表时间：
2000-09-16
影响因子：
3.1
通讯作者：
Inazawa, J
中科院分区：
文献类型：
--
作者：
Imoto, I;Pimkhaokham, A;Inazawa, J
同源域转录因子在指导细胞增殖和分化中发挥重要作用。一种 TALE 超类同源域蛋白，TGFβ 诱导转录的多功能阻遏蛋白。在这里，我们报告了 TGIF2 的鉴定，TGIF2 是一种新型 TALE 超类同源域蛋白，与 TGIF 显示出明显的同源性，特别是在其 DNA 结合域中。 TGIF2 在人体组织中广泛表达，其中在心脏、肾脏和睾丸中表达水平最高。 TGIF2产物含有假定的核定位信号；通过转染带表位标记的 cDNA，TGIF2 位于染色体 20q11.2-12 上，证实了蛋白质易位至细胞核。由于在卵巢癌中经常观察到 20q 扩增，因此我们确定了 14 个卵巢癌细胞系中 DNA 拷贝数和 TGIF2 表达的状态。通过 FISH 分析，该基因在所有显示扩增的细胞系中均过表达。结果表明TGIF2可能通过基因扩增机制在一些卵巢肿瘤的发生和/或进展中发挥重要作用。 (C) 2000 年学术出版社。
Homeodomain transcription factors play important roles in directing cellular proliferation and differentiation. A TALE-superclass homeodomain protein, multifunctional repressor of TGF beta-induced transcription. Here we report identification of TGIF2, a novel TALE-superclass homeodomain protein that shows distinct homology with TGIF, especially in its DNA-binding domain. TGIF2 is expressed ubiquitously in human tissues, with the highest levels being found in heart, kidney, and testis. The TGIF2 product contains a putative nuclear localization signal; translocation of the protein to the nucleus was confirmed by transfection of epitope-tagged cDNA, TGIF2 lies on chromosome 20q11.2-12. Since amplification of 20q is often observed among ovarian cancers, we determined the status of DNA copy-number and expression of TGIF2 in 14 ovarian-cancer cell lines, This gene was overexpressed in all lines that showed amplification by FISH analysis. The results suggested that TGIF2 may play an important role in the development and/or progression of some ovarian tumors through a mechanism of gene amplification. (C) 2000 Academic Press.