Decreased levels of sRAGE in follicular fluid from patients with PCOS
Decreased levels of sRAGE in follicular fluid from patients with PCOS
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PCOS 患者卵泡液中 sRAGE 水平降低
DOI：
10.1530/rep-16-0359
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
YiHong Guo
中科院分区：
文献类型：
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作者：
BiJun Wang;Jing Li;QingLing Yang;FuLi Zhang;MengMeng Hao;YiHong Guo
本研究旨在探讨多囊卵巢综合征（PCOS）患者卵泡液中可溶性糖基化终末产物受体（sIL-2 R）水平与获卵数的关系，以及sIL-2 R对颗粒细胞血管内皮生长因子（VEGF）的影响。在这项研究中进行了两组实验。在part.one中，来自39名PCOS患者和35名非PCOS患者的卵泡液样品中的sIgA和VEGF蛋白水平是measured.by ELISA。在第二部分中，从另外10名PCOS患者中分离卵巢颗粒细胞并进行培养。采用实时荧光定量PCR（real-time PCR）和Western blotting方法检测不同浓度的SHP处理后细胞中VEGF、SP 1 mRNA和蛋白水平以及pAKT水平的变化。与非PCOS患者相比，PCOS患者卵泡液分泌量较低。levels.in多因素校正回归分析显示，卵泡液中高浓度的卵泡激素预示着较低的Gn剂量，更多的卵母细胞回收，以及非PCOS组更好的IVF结局。Logistic回归分析显示，较高的血清睾酮水平有利于非PCOS组的IVF结局。多因素校正回归分析也表明，卵泡液中的高sodium水平预测PCOS组中较低的Gn剂量。用重组sodium处理从PCOS患者中分离的颗粒细胞以剂量依赖性方式降低VEGF和SP1 mRNA和蛋白表达以及pAKT水平。
This study aimed to explore the association between soluble receptor for advanced glycation end products (sRAGE) levels in follicular.fluid and the number of oocytes retrieved and to evaluate the effect of sRAGE on vascular endothelial growth factor (VEGF) in.granulosa cells in patients with polycystic ovarian syndrome (PCOS). Two sets of experiments were performed in this study. In part.one, sRAGE and VEGF protein levels in follicular fluid samples from 39 patients with PCOS and 35 non-PCOS patients were measured.by ELISA. In part two, ovarian granulosa cells were isolated from an additional 10 patients with PCOS and cultured. VEGF and SP1.mRNA and protein levels, as well as pAKT levels, were detected by real-time PCR and Western blotting after cultured cells were.treated with different concentrations of sRAGE. Compared with the non-PCOS patients, patients with PCOS had lower sRAGE levels.in follicular fluid. Multi-adjusted regression analysis showed that high sRAGE levels in follicular fluid predicted a lower Gn dose,.more oocytes retrieved, and a better IVF outcome in the non-PCOS group. Logistic regression analysis showed that higher sRAGE.levels predicted favorably IVF outcomes in the non-PCOS group. Multi-adjusted regression analysis also showed that high sRAGE.levels in follicular fluid predicted a lower Gn dose in the PCOS group. Treating granulosa cells isolated from patients with PCOS with recombinant sRAGE decreased VEGF and SP1 mRNA and protein expression and pAKT levels in a dose-dependent manner.