Cryopreservation and its clinical applications.
Cryopreservation and its clinical applications.
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DOI：
10.1016/j.imr.2016.12.001
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发表时间：
2017-03
影响因子：
3.4
通讯作者：
Han J
中科院分区：
文献类型：
--
作者：
Jang TH;Park SC;Yang JH;Kim JY;Seok JH;Park US;Choi CW;Lee SR;Han J
冷冻保存是通过将样品冷却至非常低的温度来保存细胞器、细胞、组织或任何其他生物构造的过程。活细胞对冰形成的反应具有理论意义和实际意义。干细胞和其他有活力的组织在基础研究以及许多医学应用中具有巨大的潜力，不能通过简单的冷却或冷冻长时间储存，因为在冷冻和解冻过程中冰晶形成、渗透压休克和膜损伤将导致细胞死亡。近年来，随着冷冻保护剂和温度控制设备的使用，细胞和组织的成功冷冻保存逐渐增加。持续了解冻融循环中发生的物理和化学性质对于细胞或组织的成功冷冻保存及其临床应用是必要的。在这篇综述中，我们简要地讨论了代表性的低温保存过程，如缓慢冷冻和玻璃化，以及可用的冷冻保护剂。此外，还提到了冷冻保存的一些不利影响。
Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures. The responses of living cells to ice formation are of theoretical interest and practical relevance. Stem cells and other viable tissues, which have great potential for use in basic research as well as for many medical applications, cannot be stored with simple cooling or freezing for a long time because ice crystal formation, osmotic shock, and membrane damage during freezing and thawing will cause cell death. The successful cryopreservation of cells and tissues has been gradually increasing in recent years, with the use of cryoprotective agents and temperature control equipment. Continuous understanding of the physical and chemical properties that occur in the freezing and thawing cycle will be necessary for the successful cryopreservation of cells or tissues and their clinical applications. In this review, we briefly address representative cryopreservation processes, such as slow freezing and vitrification, and the available cryoprotective agents. In addition, some adverse effects of cryopreservation are mentioned.