Characterization of RUSC1 and RUSC2 genes in silico.
Characterization of RUSC1 and RUSC2 genes in silico.
复制标题
RUSC1 和 RUSC2 基因的计算机表征。
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
M. Katoh
中科院分区：
文献类型：
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作者：
Masuko Katoh;M. Katoh
我们先前已经克隆（或鉴定）并表征了编码SH 3结构域蛋白的新基因，如MAP 3 K10、FNBP 1 L、FNBP 2、FCHSD 1、FCHSD 2、LASP 2和MPP 7。在SH 3结构域分子的表征过程中，我们发现存在N-末端截短的433-aa RUSC 1蛋白（NP_055143.2）。在这里，我们通过使用生物信息学来表征RUSC 1和RUSC 2基因。IMAGE 5204626（BC025680.1）和KIAA 0375（AB002373.2）分别是源自人RUSC 1和RUSC 2基因的代表性cDNA。2210403 N 08（AK039664.1）和MGC 73483（BC056360.1）cDNA来源于小鼠Rusc 1基因，而Kiaa 0375（AK122263.1）cDNA来源于小鼠Rusc 2基因。RUSC 1基因定位于人染色体1 q22，由11个外显子组成。RUSC 2基因定位于人染色体9 p13，由12个外显子组成。人和小鼠的Rusc 1基因均编码两种亚型，由于外显子跳跃型的选择性剪接，分别含有或不含有411 bp的外显子3。RUSC 1亚型1（1039 aa）和RUSC 2（1516 aa）显示30.5%的总氨基酸同一性。发现人RUSC 1、RUSC 2、小鼠Rusc 1和Rusc 2蛋白具有共同的结构域结构，由RUN、亮氨酸拉链和SH 3结构域组成。RUSC 1、RUSC 2、RIPX、RUFY 1、RUFY 2、RUTBC 1、RUTBC 2、RUTBC 3、RUB 6 IP 1和PLEKHM 1是人蛋白质组中的RUN结构域蛋白。这是关于RUSC 1和RUSC 2直系同源物的全面表征的第一份报告。
We have previously cloned (or identified) and characterized novel genes encoding SH3 domain proteins, such as MAP3K10, FNBP1L, FNBP2, FCHSD1, FCHSD2, LASP2 and MPP7. During characterization of SH3 domain molecules, we found the existence of an N-terminally- truncated 433-aa RUSC1 protein (NP_055143.2). Here, we characterized RUSC1 and RUSC2 genes by using bioinformatics. IMAGE 5204626 (BC025680.1) and KIAA0375 (AB002373.2) were representative cDNAs derived from human RUSC1 and RUSC2 genes, respectively. 2210403N08 (AK039664.1) and MGC73483 (BC056360.1) cDNAs were derived from mouse Rusc1 gene, while Kiaa0375 (AK122263.1) cDNA was derived from mouse Rusc2 gene. RUSC1 gene, consisting of 11 exons, was mapped to human chromosome 1q22. RUSC2 gene, consisting of 12 exons was mapped to human chromosome 9p13. Human RUSC1 gene as well as mouse Rusc1 gene were found to encode two isoforms with or without 411-bp exon 3 due to alternative splicing of exon-skipping type. RUSC1 isoform 1 (1039 aa) and RUSC2 (1516 aa) showed 30.5% total amino-acid identity. Human RUSC1, RUSC2, mouse Rusc1 and Rusc2 proteins were found to share the common domain structure, consisting of RUN, Leucine zipper, and SH3 domains. RUSC1, RUSC2, RIPX, RUFY1, RUFY2, RUTBC1, RUTBC2, RUTBC3, RUB6IP1, and PLEKHM1 were RUN domain proteins within the human proteome. This is the first report on comprehensive characterization of RUSC1 and RUSC2 orthologs.