Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice.
Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice.
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通过用N，M和E基因诱导DNA疫苗对小鼠SARS-COV的DNA疫苗接种。
DOI：
10.1016/j.bbrc.2005.01.048
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发表时间：
2005-03-25
影响因子：
3.1
通讯作者：
Wang B
中科院分区：
文献类型：
--
作者：
Jin H;Xiao C;Chen Z;Kang Y;Ma Y;Zhu K;Xie Q;Tu Y;Yu Y;Wang B
针对 SARS-CoV 感染的疫苗接种是控制病毒在公共场所传播的一种有吸引力的手段。在这项研究中，我们采用了 DNA 疫苗技术和左旋咪唑（我们新发现的化学佐剂）来产生 Th1 型反应。为了避免增强抗体问题，克隆了编码 SARS-CoV 核衣壳、膜和包膜蛋白的基因，并测定了它们在哺乳动物细胞中的表达。肌肉注射到动物体内后，我们观察到E、M和N基因的构建体可以诱导高水平的特异性抗体、T细胞增殖、IFN-γ、DTH反应以及体内针对SARS-CoV抗原的细胞毒性T细胞活性。编码核衣壳蛋白的构建体产生了最高的免疫反应。结果表明，N、M和E基因可以作为DNA疫苗开发中预防SARS-CoV感染的靶点。
Vaccination against the SARS-CoV infection is an attractive means to control the spread of viruses in public. In this study, we employed a DNA vaccine technology with the levamisole, our newly discovered chemical adjuvant, to generate Th1 type of response. To avoid the enhancement antibody issue, genes encoding the nucleocapsid, membrane, and envelope protein of SARS-CoV were cloned and their expressions in mammalian cells were determined. After the intramuscular introduction into animals, we observed that the constructs of the E, M, and N genes could induce high levels of specific antibodies, T cell proliferations, IFN-γ, DTH responses, and in vivo cytotoxic T cells activities specifically against SARS-CoV antigens. The highest immune responses were generated by the construct encoding the nucleocapsid protein. The results suggest that the N, M, and E genes could be used as the targets to prevent SARS-CoV infection in the DNA vaccine development.
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影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
影响因子：
2.2
作者：
REINER, SL;ZHENG, SC;LOCKSLEY, RM
通讯作者：
LOCKSLEY, RM
影响因子：
5.5
作者：
Chen, Z;Matsuo, K;Tamura, S
通讯作者：
Tamura, S
影响因子：
3.8
作者：
Glansbeek，HL;Haagmans，BL;Rottier，PJM
通讯作者：
Rottier，PJM
影响因子：
5.4
作者：
Kiszka, I;Kmieciak, D;Kozbor, D
通讯作者：
Kozbor, D