A NEW SULFONATED TETRAZOLIUM SALT THAT PRODUCES A HIGHLY WATER-SOLUBLE FORMAZAN DYE
A NEW SULFONATED TETRAZOLIUM SALT THAT PRODUCES A HIGHLY WATER-SOLUBLE FORMAZAN DYE
复制标题
DOI：
10.1248/cpb.41.1118
复制
发表时间：
1993-06-01
影响因子：
1.7
通讯作者：
HE, PG
中科院分区：
文献类型：
--
作者：
ISHIYAMA, M;SHIGA, M;HE, PG
合成了一种新的四唑化合物，即 4-[3-(4-碘苯基)-2-(4-硝基苯基)-2H-5-四唑]-1,3-苯二磺酸 (1a) 的钠盐，由于存在两个磺酸盐基团，该化合物可产生高度水溶性的甲臜染料，并评估了其在 NADH 和细胞增殖测定中的潜在实用性。该化合物在 NADH 测定中被证明对 2,3-双(2-甲氧基-4-硝基-5-磺基苯基)-5-[(苯基氨基)羰基]-2H-氢氧化四唑 (XTT) 具有相似的敏感性，并且在使用 P388 细胞系的增殖测定中也可用作细胞活力的指示剂，其细胞毒性低于 XTT。
A new tetrazolium compound, sodium salt of 4-[3-(4-iodophenyl)-2-(4-nitropbenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (1a), which produces a highly water-soluble formazan dye, due to the presence of two sulfonate groups, was synthesized and its potential utility evaluated in assays of NADH and cell proliferation. The compound proved to have a similar sensitivity to 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) in the assay of NADH and was also useful as an indicator of cell viability, with less cytotoxicity than XTT, in the proliferation assay using P388 cell lines.