Quantitative analysis of small molecule-nucleic acid interactions with a biosensor surface and surface plasmon resonance detection.
Quantitative analysis of small molecule-nucleic acid interactions with a biosensor surface and surface plasmon resonance detection.
复制标题
DOI：
10.1007/978-1-60327-418-0_1
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Wilson, W David
中科院分区：
文献类型：
--
作者：
Liu, Yang;Wilson, W David
表面等离子体共振（SPR）技术与生物传感器表面已成为一种广泛使用的工具，用于研究核酸相互作用，没有任何标记的要求。该方法提供了同时的动力学和平衡特性的生物分子的相互作用，以及小分子生物聚合物结合。SPR监测分子相互作用在真实的时间，并提供了显着的优势，光学或量热方法的系统与强结合耦合小的光谱信号和/或反应热。详细和实用的指南核酸相互作用分析使用SPR生物传感器的方法。详细的SPR技术和基本原理的SPR仪器，传感器芯片和样品的准备，以及实验设计，定量和定性数据分析和演示的广泛信息的建议。一个具体的例子与DNA的小沟结合剂的相互作用的动力学和稳态SPR方法来说明该技术进行评估。由于与特定DNA序列协同结合的分子在许多应用中具有吸引力，因此也提出了协同小分子-DNA相互作用。
Surface plasmon resonance (SPR) technology with biosensor surfaces has become a widely–used tool for the study of nucleic acid interactions without any labeling requirements. The method provides simultaneous kinetic and equilibrium characterization of the interactions of biomolecules as well as small molecule–biopolymer binding. SPR monitors molecular interactions in real time and provides significant advantages over optical or calorimetic methods for systems with strong binding coupled with small spectroscopic signals and/or reaction heats. A detailed and practical guide for nucleic acid interaction analysis using SPR–biosensor methods is presented. Details of the SPR technology and basic fundamentals are described with recommendations on the preparation of the SPR instrument, sensor chips and samples as well as extensive information on experimental design, quantitative and qualitative data analysis and presentation. A specific example of the interaction of a minor–groove–binding agent with DNA is evaluated by both kinetic and steady–state SPR methods to illustrate the technique. Since the molecules that bind cooperatively to specific DNA sequences are attractive for many applications, a cooperative small molecule–DNA interaction is also presented.