The Arabidopsis AGC kinases NDR2/4/5 interact with MOB1A/1B and play important roles in pollen development and germination
The Arabidopsis AGC kinases NDR2/4/5 interact with MOB1A/1B and play important roles in pollen development and germination
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DOI：
10.1111/tpj.15085
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发表时间：
2020-12-11
期刊：
影响因子：
7.2
通讯作者：
Ye, De
中科院分区：
文献类型：
--
作者：
Zhou, Peng-Min;Liang, Yan;Ye, De
花粉的形成和花粉管的生长是雄配子进入雌胚囊进行双受精的关键。关于花粉形成和萌发的后期发育过程的调控机制知之甚少。在这项研究中，我们的特点是一组拟南芥AGC激酶蛋白，NDR 2/4/5，参与花粉发育和花粉萌发。NDR 2/4/5基因主要在发育后期的花粉粒和花粉管中表达。它们在花粉形成和花粉萌发中起着冗余的作用。在三细胞时期，ndr 2 ndr 4 ndr 5突变体花粉粒表现出胼胝质异常积累、花药早熟萌发和破裂，导致受精率急剧下降和结实率降低。NDR 2/4/5蛋白可以与另一组蛋白（MOB 1A/1B）相互作用，这些蛋白与酵母和动物中Hippo信号通路的MOB蛋白同源。拟南芥mob 1a mob 1b突变体花粉粒也具有与ndr 2 ndr 4 ndr 5花粉粒相似的表型。这些结果为证明Hippo信号通路在植物中的保守性和在植物有性生殖中的重要作用提供了新的证据。
Pollen formation and pollen tube growth are essential for the delivery of male gametes into the female embryo sac for double fertilization. Little is known about the mechanisms that regulate the late developmental process of pollen formation and pollen germination. In this study, we characterized a group of Arabidopsis AGC kinase proteins, NDR2/4/5, involved in pollen development and pollen germination. The NDR2/4/5 genes are mainly expressed in pollen grains at the late developmental stages and in pollen tubes. They function redundantly in pollen formation and pollen germination. At the tricellular stages, the ndr2 ndr4 ndr5 mutant pollen grains exhibit an abnormal accumulation of callose, precocious germination and burst in anthers, leading to a drastic reduction in fertilization and a reduced seed set. NDR2/4/5 proteins can interact with another group of proteins (MOB1A/1B) homologous to the MOB proteins from the Hippo signaling pathway in yeast and animals. The Arabidopsis mob1a mob1b mutant pollen grains also have a phenotype similar to that of ndr2 ndr4 ndr5 pollen grains. These results provide new evidence demonstrating that the Hippo signaling components are conserved in plants and play important roles in sexual plant reproduction.