Beauveria bassiana: a new N(6)-(2-hydroxyethyl)-adenosine-producing fungus.
Beauveria bassiana: a new N(6)-(2-hydroxyethyl)-adenosine-producing fungus.
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白僵菌：一种新的 N6-(2-羟乙基)-腺苷生产真菌
DOI：
10.1080/21501203.2017.1375040
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Dong C
中科院分区：
文献类型：
--
作者：
Liu K;Wang F;Wang W;Dong C
摘要N6-（2-羟乙基）-腺苷（HEA）是第一个生物来源的钙拮抗剂，由于其具有抑制肿瘤细胞增殖、抑制炎症、保护肾脏以及镇静和杀虫等作用而受到广泛研究。到目前为止，仅在少数几种虫草中检测到HEA的产生，如粉霜虫草、粉蛹虫草等。militaris和Isaria tenuipes。在此，我们发现了一种新的产HEA的真菌，根据形态学和系统发育特征，将其鉴定为球孢白僵菌。HEA的生产进行了验证，通过反相高效液相色谱/质谱在该真菌。此外，在另外两株B的菌丝体中也检测到HEA。来自不同来源的白僵菌菌株，但不是在所有测试的B的培养基中。球孢菌株在第7天达到HEA的最大产量（0.8483 ± 0.0439 mg/g菌丝体干重）。真菌生物量和HEA生产动力学在培养期间表现出类似的趋势。这是描述B中HEA生产的第一份报告。这表明该真菌菌株可能具有作为HEA来源的新应用。
ABSTRACT N6-(2-Hydroxyethyl)-adenosine (HEA), which was the first calcium antagonist derived from biological sources, has been intensively investigated because of its ability to inhibit tumour cell proliferation, restrain inflammation, protect kidneys and function as a sedative and insecticide. Up to now, the production of HEA has been detected only in a few species such as Cordyceps pruinosa, C. militaris and Isaria tenuipes. Here, we found a new HEA-producing fungus, which was identified as Beauveria bassiana based on morphological and phylogenetic characteristics. The HEA production was verified by reversed-phase high-performance liquid chromatography/mass spectrometry in this fungus. Moreover, HEA was also detected in the mycelia of two other B. bassiana strains from different origins, but not in the culture medium of all tested B. bassiana strains. The maximum production of HEA (0.8483 ± 0.0439 mg/g mycelia dry weight) was achieved on day 7. The fungal biomass and kinetics of HEA production exhibited similar trends over the duration of the culture period. This is the first report describing HEA production in B. bassiana, which suggests that this fungal strain may have new applications as a source of HEA.