INTERACTIONS BETWEEN ARBACIA SPERM AND S35—LABELLED FERTILIZIN
INTERACTIONS BETWEEN ARBACIA SPERM AND S35—LABELLED FERTILIZIN
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ARBACIA 精子与 S35 标记的肥料之间的相互作用
DOI：
10.2307/1539538
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发表时间：
1961
期刊：
影响因子：
--
通讯作者：
C. B. Metz
中科院分区：
文献类型：
--
作者：
R. R. Hathaway;C. B. Metz
1.本文用S ~（35）标记的受精素研究了阿氏藻受精素与精子的反应。实验包括将受精卵和精子混合，从悬浮液中分离精子，并分析上清液的放射性。用这种方法，精子对受精素的吸收可以用上清液中放射性的损失来测量。10项实验的结果表明，除28%外，所有受精卵都被精子初步吸收。这是迅速其次是释放超过一半的吸收材料。3.与受精素发生反应的精子在进一步用这种物质处理后不再吸收额外的受精素。与精子发生反应的受精液不会被新鲜精子明显吸收。透析结果表明，S35标记物质在精子中的吸收和释放是不扩散的。三种异育性精子吸收Arbacia fertilizin，但不释放.吸收和...
1. Reactions between Arbacia fertilizin and sperm were studied with preparations of S35-labelled fertilizin. Experiments consisted of mixing fertilizin and sperm, centrifuging the sperm from the suspension, and assaying the supernatant for radioactivity. In this way the absorption of fertilizin to sperm could be measured as a loss of radioactivity from the supernatant.2. Results of 10 experiments show that there was an initial absorption by sperm of all but 28% of the fertilizin. This was rapidly followed by a release of more than one-half the absorbed material.3. Sperm which have reacted with fertilizin do not absorb additional fertilizin upon further treatment with this substance.4. Fertilizin solutions which have reacted with sperm are not appreciably absorbed by fresh sperm.5. Dialysis shows that the S35-labelled material absorbed to and released from sperm is non-diffusible.6. Heterologous sperm from three species absorb Arbacia fertilizin but do not release it.7. The parallel between absorption and ...