Cutting Edge: Cross-Presentation of Cell-Associated Antigens to MHC Class I Molecule Is Regulated by a Major Transcription Factor for Heat Shock Proteins1
Cutting Edge: Cross-Presentation of Cell-Associated Antigens to MHC Class I Molecule Is Regulated by a Major Transcription Factor for Heat Shock Proteins1
复制标题
前沿：MHC I 类分子的细胞相关抗原的交叉呈递受热休克蛋白主要转录因子的调节1
作者：
Hong Zheng;Zihai Li
专业APC从实质细胞向MHC-I类抗原交叉递送的能力对于启动和CD8+T细胞对细胞内AGS的耐受性是必不可少的。由于非细胞相关的游离AGS的交叉呈现是低效的，分子伴侣或热休克蛋白(HSPs)在AGS到APC的伴侣中的作用已被推测。在这里，我们使用热休克因子1(HSF1)缺陷的小鼠从基因上解决了这一假设，热休克因子1是热休克蛋白的主要转录因子。热休克蛋白1−/−小鼠体内热休克蛋白90和热休克蛋白70等多种热休克蛋白表达降低。使用多种抗原系统，我们证明了当抗原表达限于HSF1−/−非抗原前体细胞时，交叉激发抗原特异性的CD8+T细胞是无效的。我们的研究为HSF1在调节MHC I类相关AGS的交叉呈现中的作用提供了第一个遗传学证据。
The ability for the professional APC to cross-present Ag to MHC class I from parenchymal cells is essential for priming as well as tolerance of CD8+ T cells against intracellular Ags. Since cross-presentations of non-cell-associated free Ags are inefficient, the roles of molecular chaperones or heat shock proteins (HSPs) in chaperoning Ags to APCs have been postulated. We herein genetically addressed this hypothesis using mice that were defective of heat shock factor 1 (Hsf1), a major transcription factor for HSPs. Hsf1−/− mice have a decreased expression of several HSPs including HSP90 and HSP70. Using multiple Ag systems, we demonstrated that cross-priming of Ag-specific CD8+ T cells was inefficient when Ag expression was restricted to Hsf1−/− non-APCs. Our study provides the first genetic evidence for the roles of Hsf1 in regulating cross-presentation of MHC class I-associated Ags.
影响因子：
11.4
作者：
LI, ZH;SRIVASTAVA, PK
通讯作者：
SRIVASTAVA, PK
影响因子：
56.9
作者：
SUTO, R;SRIVASTAVA, PK
通讯作者：
SRIVASTAVA, PK