Role of Kruppel homolog 1 (Kr-h1) in methyl farnesoate-mediated vitellogenesis in the swimming crab Portunus trituberculatus
Role of Kruppel homolog 1 (Kr-h1) in methyl farnesoate-mediated vitellogenesis in the swimming crab Portunus trituberculatus
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Kruppel 同源物 1 (Kr-h1) 在法呢酸甲酯介导的三疣梭子蟹卵黄发生中的作用
DOI：
10.1016/j.gene.2018.08.046
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Dongfa Zhu
中科院分区：
文献类型：
--
作者：
Xi Xie;Mingxin Liu;Qinghua Jiang;Hongkun Zheng;Liang Zheng;Dongfa Zhu
与幼体激素（JH）在昆虫中的作用类似，法尼索酸甲酯（MF）， JH III的未环氧化形式，调节甲壳类动物的许多发育过程，如换壳和繁殖。我们之前已经表明，JH受体，耐甲氧苯醚（Met），也是MF的候选受体，可能参与MF介导的游泳蟹Portunus trituberculatus的卵黄形成。本研究进一步研究了Met下游转录因子Kruppel同源物1 （Kruppel homolog 1, Kr-h1）在JH信号传导中的作用。推断出的Pt-Kr-h1蛋白含有7个重复的锌指基序，与其他甲壳类动物的Kr-h1s相似，但与昆虫Kr-h1s中的8个锌指基序不同。体外MF处理诱导肝胰腺中pt - kr -h1的表达，而卵巢中没有，这与之前报道的pt - metas的MF反应模式相似。此外，用pt - metdsrna治疗后，Pt-Kr-h1的表达显著降低，这强烈表明Pt-Kr-h1可能参与met介导的MF信号通路。pt - metandpt -Kr-h1的RNAi均导致卵黄蛋白原（vitellogenin, Vg）的表达减少，而添加MF无法挽救这种减少，提示MF对卵黄蛋白形成的调控可能通过Met和Kr-h1起作用。这些结果将有助于加深对MF信号调控机制的认识，并为进一步研究节肢动物激素通路的进化提供重要资源。
Similar to the role of juvenile hormone (JH) in insects, methyl farnesoate (MF), the unepoxidized form of JH III, regulates many developmental processes in crustaceans, such as molting and reproduction. We have previously showed that the JH receptor, Methoprene-tolerant (Met), which is also a candidate receptor for MF, might be involved in the MF-mediated vitellogenesis in the swimming crab Portunus trituberculatus. In this study, the role of Kruppel homolog 1 (Kr-h1), a transcription factor downstream Met in JH signaling, was further investigated. The deduced protein of Pt-Kr-h1 contained seven repeats of zinc finger motifs, similar to Kr-h1s from other crustacean species, but differing from the eight zinc finger motifs found in insect Kr-h1s. MF treatmentin vitroinduced the expression ofPt-Kr-h1in hepatopancreas but not ovary, which is similar to the MF-responsive pattern ofPt-Metas previously reported. Moreover, the expression ofPt-Kr-h1decreased significantly after treating withPt-MetdsRNA, strongly indicating that the Pt-Kr-h1 might be involved in the Met-mediated MF signaling pathway. RNAi ofPt-MetandPt-Kr-h1both led to a decrease in vitellogenin (Vg) expression, and the reduction cannot be rescued by adding MF, suggesting the regulation of vitellogenesis by MF may act through Met and Kr-h1. These results would help to enhance the current understanding of the regulatory mechanism of MF signaling, and provide a vital resource for further research into the evolution of hormonal pathways in arthropods.