Dysbiosis of Gut Microbiota Associated with Clinical Parameters in Polycystic Ovary Syndrome.
Dysbiosis of Gut Microbiota Associated with Clinical Parameters in Polycystic Ovary Syndrome.
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肠道菌群失调与多囊卵巢综合征临床参数相关
DOI：
10.3389/fmicb.2017.00324
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发表时间：
2017
影响因子：
5.2
通讯作者：
Ding X
中科院分区：
文献类型：
--
作者：
Liu R;Zhang C;Shi Y;Zhang F;Li L;Wang X;Ling Y;Fu H;Dong W;Shen J;Reeves A;Greenberg AS;Zhao L;Peng Y;Ding X
多囊卵巢综合征（PCOS）是一种常见的女性内分泌代谢紊乱疾病。肠道微生物群在代谢性疾病中起关键作用，并可能调节脑-肠轴介质的分泌。肠道菌群与PCOS的内分泌和生化紊乱之间的相互作用仍然是难以捉摸的。在这里，我们发现改变的肠道微生物群与PCOS表型显著相关。有33例PCOS患者（非肥胖PCOS个体，PN，n = 12;肥胖PCOS个体，PO，n = 21）以及15例对照受试者（非肥胖对照个体，CN，n = 9;肥胖对照个体，CO，n = 6）入组我们的研究。PCOS患者血浆5-羟色胺、生长激素释放肽和肽YY（PYY）水平较对照组显著降低，且与腰围、睾酮呈显著负相关。粪便样本中16 S rRNA基因V3-V4区的测序揭示了PCOS和非肥胖对照之间肠道微生物物种的实质性差异。根据细菌相对丰度的SparCC相关系数，将细菌种分为23个共丰度组（CAG）。PCOS患者CAG升高，包括拟杆菌属、大肠杆菌/志贺氏菌属和链球菌属，与ghrelin呈负相关，与睾酮、BMI呈正相关。PCOS患者CAG的降低与体重、性激素、脑肠肽呈负相关，包括阿克曼氏菌和瘤胃球菌。总之，多囊卵巢综合征患者的肠道微生物生态失调与疾病表型相关。
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. Gut microbiota has been implicated to play a critical role in metabolic diseases and may modulate the secretion of mediators of the brain–gut axis. Interaction between gut microbiota and the endocrine and biochemical disturbances in PCOS still remains elusive. Here, we showed an altered gut microbiota significantly correlated with PCOS phenotype. There were 33 patients with PCOS (non-obese PCOS individuals, PN, n = 12; obese PCOS individuals, PO, n = 21) as well as 15 control subjects (non-obese control individuals, CN, n = 9; obese control individuals, CO, n = 6) enrolled in our study. The plasma levels of serotonin, ghrelin, and peptide YY (PYY) were significantly decreased in patients with PCOS compared with controls, and have a significantly negative correlation with waist circumference and testosterone. Sequencing of the V3–V4 region of the 16S rRNA gene in fecal samples revealed the substantial differences of gut microbial species between the PCOS and non-obese controls. Bacterial species were clustered into 23 co-abundance groups (CAGs) based on the SparCC correlation coefficients of their relative abundance. The CAGs increased in PCOS, including the bacteria belonging to Bacteroides, Escherichia/Shigella and Streptococcus, were negatively correlated with ghrelin, and positively correlated with testosterone and BMI. Furthermore, the CAGs that were decreased in PCOS, including the bacteria from Akkermansia and Ruminococcaceae, showed opposite relationship with body-weight, sex-hormone, and brain–gut peptides. In conclusion, gut microbial dysbiosis in women with PCOS is associated with the disease phenotypes.