Microbial interactions and differential protein expression in Staphylococcus aureus -Candida albicans dual-species biofilms.
Microbial interactions and differential protein expression in Staphylococcus aureus -Candida albicans dual-species biofilms.
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DOI：
10.1111/j.1574-695x.2010.00710.x
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发表时间：
2010-08
影响因子：
--
通讯作者：
Shirtliff ME
中科院分区：
文献类型：
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作者：
Peters BM;Jabra-Rizk MA;Scheper MA;Leid JG;Costerton JW;Shirtliff ME
真菌种白色念珠菌和细菌种金黄色葡萄球菌是大多数医院获得性感染的原因，并经常合并感染危重病人作为复杂的多菌生物膜。为了研究多菌体生长过程中的生物膜结构，用共聚焦扫描激光显微镜对双物种生物膜进行了成像。分析揭示了一种独特的生物膜结构，其中金黄色葡萄球菌通常与白色念珠菌的菌丝成分有关。这种物理相互作用可能为葡萄球菌提供一种侵袭策略，因为念珠菌菌丝可以穿透上皮层。为了进一步了解可能导致先前证实的在混合感染期间放大毒力的分子机制，进行了蛋白质表达研究。差示凝胶内电泳法鉴定了这些生物在共培养生物膜生长过程中显著差异产生的27种蛋白质。在上调的葡萄球菌蛋白中，有L-乳酸脱氢酶1，它能抵抗宿主来源的氧化应激源。在下调的蛋白质中，有一种是毒力因子的全球转录抑制因子Cody。这些发现表明，菌丝介导的金黄色葡萄球菌的增强致病作用可能不仅是由于物理上的相互作用，还可以归因于在多菌生长过程中诱导的特定毒力因子的不同调节。有必要在分子水平上进一步描述这些病原体之间错综复杂的相互作用，因为这可能有助于设计旨在对抗真菌-细菌多菌感染的新的治疗策略。
The fungal species Candida albicans and the bacterial species Staphylococcus aureus are responsible for a majority of hospital-acquired infections and often coinfect critically ill patients as complicating polymicrobial biofilms. To investigate biofilm structure during polymicrobial growth, dual-species biofilms were imaged with confocal scanning laser microscopy. Analyses revealed a unique biofilm architecture where S. aureus commonly associated with the hyphal elements of C. albicans. This physical interaction may provide staphylococci with an invasion strategy because candidal hyphae can penetrate through epithelial layers. To further understand the molecular mechanisms possibly responsible for previously demonstrated amplified virulence during coinfection, protein expression studies were undertaken. Differential in-gel electrophoresis identified a total of 27 proteins to be significantly differentially produced by these organisms during coculture biofilm growth. Among the upregulated staphylococcal proteins was l-lactate dehydrogenase 1, which confers resistance to host-derived oxidative stressors. Among the downregulated proteins was the global transcriptional repressor of virulence factors, CodY. These findings demonstrate that the hyphae-mediated enhanced pathogenesis of S. aureus may not only be due to physical interactions but can also be attributed to the differential regulation of specific virulence factors induced during polymicrobial growth. Further characterization of the intricate interaction between these pathogens at the molecular level is warranted, as it may aid in the design of novel therapeutic strategies aimed at combating fungal–bacterial polymicrobial infection.