IL-12 in conjunction with dendritic cells enhances antiviral CD8+ CTL responses in vitro
IL-12 in conjunction with dendritic cells enhances antiviral CD8+ CTL responses in vitro
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DOI：
10.1172/jci118843
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发表时间：
1996-08-01
影响因子：
15.9
通讯作者：
Feldman, MV
中科院分区：
文献类型：
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作者：
Bhardwaj, N;Seder, RA;Feldman, MV
CD8+细胞溶解T淋巴细胞（ctl）是抵抗感染和恶性疾病的重要介质。IL-12增强杀伤细胞的增殖和细胞溶解反应，但其在产生人类抗病毒CD8+ T细胞反应中的功能尚未明确。因此，我们评估了IL-12在流感感染树突状细胞生成ctl中的作用。IL-12在感染树突状细胞的上清液中或CTL生成过程中检测不到。此外，抗il -12抗体不阻断CTL的产生。然而，外源性IL-12 （30-300 pg/ml）增强了CD8+ T细胞的增殖和细胞溶解反应。对流感病毒反应性弱的个体或抗原提呈细胞（APC）：T细胞比例为1:100或更小的个体效果最大。IL-12增强了CTL生成过程中干扰素的产生。然而，抗干扰素抗体不能阻断细胞因子对CTL的增强作用。与IL-12一起，抗原脉冲树突状细胞可能是增强CTL对感染因子和恶性肿瘤反应的有效方法。
CD8+ cytolytic T lymphocytes (CTLs) are important mediators for resistance to infections and malignant diseases. IL-12 enhances proliferative and cytolytic responses by killer cells, but its function in the generation of human antiviral CD8+ T cell responses has not been defined. We therefore evaluated the role of IL-12 in the generation of CTLs to influenza-infected dendritic cells. IL-12 was not detectable in supernatants of infected-dendritic cells, or during CTL generation. Furthermore, anti-IL-12 antibody did not block CTL generation. However, exogenous IL-12 (30-300 pg/ml) enhanced CD8+ T cell proliferative and cytolytic responses. The effect was greatest in individuals with weak reactivity to influenza virus or at antigen-presenting cell (APC):T cell ratios of 1:100 or less. IL-12 augmented interferon-gamma production during CTL generation. The CTL enhancing effects of the cytokine, however, could not be blocked by neutralizing anti-interferon-gamma antibody. Together with IL-12, antigen-pulsed dendritic cells may be a useful approach for boosting CTL responses against infectious agents and malignancies.