Comparison of bilaterally 6-OHDA- and MPTP-lesioned rats as models of the early phase of Parkinson's disease: Histological, neurochemical, motor and memory alterations
Comparison of bilaterally 6-OHDA- and MPTP-lesioned rats as models of the early phase of Parkinson's disease: Histological, neurochemical, motor and memory alterations
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DOI：
10.1016/j.jneumeth.2005.04.005
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发表时间：
2005-10-15
影响因子：
3
通讯作者：
Da Cunha, C
中科院分区：
文献类型：
--
作者：
Ferro, MM;Bellissimo, MI;Da Cunha, C
本研究比较了两种帕金森病模型的组织学、神经化学、行为、运动和认知改变以及死亡率，其中将100 μ g 1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）或6 μ g 6-羟基多巴胺（6-OHDA）双侧注入成年雄性Wistar大鼠黑质致密部的中央区域。这两种神经毒素引起黑质酪氨酸羟化酶免疫染色细胞和纹状体多巴胺耗竭的显着损失，但6-OHDA引起更广泛和激烈的细胞损失，更激烈的体重减轻和死亡率比MPTP。6-OHDA和MPTP损伤大鼠表现出类似的缺陷，在执行工作记忆和提示版本的Morris水迷宫任务和一些探索/运动的变化，在开放领域和僵硬症测试。然而，MPTP损伤后大鼠的自发活动出现显著和短暂的增加，6-OHDA损伤后倾向于出现运动功能减退行为。在MPTP损伤的大鼠模型中观察到的轻度运动效应和习惯学习和空间工作记忆的强烈损害的图片是帕金森病的早期阶段。(c)2005 Elsevier B. V.保留所有权利。
This study compares histological, neurochemical, behavioral, motor and cognitive alterations as well as mortality of two models of Parkinson's disease in which 100 mu g 1-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine (MPTP) or 6 mu g 6-hydroxydopamine (6-OHDA) was bilaterally infused into the central region of the substantia nigra, compact part, of adult male Wistar rats. Both neurotoxins caused a significant loss of nigral tyrosine hydroxylase-immunostained cells and striatal dopamine depletion, but 6-OHDA caused more widespread and intense cell loss, more intense body weight loss and more mortality than MPTP. Both 6-OHDA- and MPTP-lesioned rats presented similar deficits in performing a working memory and a cued version of the Morris water maze task and few exploratory/motor alterations in the open field and catalepsy tests. However, rats presented a significant and transitory increase in locomotor activity after the MPTP lesion and a hypolocomotor behavior tended to be present after the 6-OHDA lesion. The picture of mild motor effects and robust impairment of habit learning and spatial working memory observed in MPTP-lesioned rats models the early phase of Parkinson's disease. (c) 2005 Elsevier B.V. All rights reserved.