Light sheet fluorescence microscopy using high-speed structured and pivoting illumination
Light sheet fluorescence microscopy using high-speed structured and pivoting illumination
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DOI：
10.1364/ol.41.005015
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发表时间：
2016-11-01
期刊：
影响因子：
3.6
通讯作者：
Solgaard, Olav
中科院分区：
文献类型：
--
作者：
Itoh, Ryosuke;Landry, Joseph Russell;Solgaard, Olav
我们发现，具有结构和枢轴照明的光片荧光显微镜能够快速获取图像并提高图像质量。利用一维空间光相位调制器实现了对照明轮廓的高速控制。为了演示该系统的特点，我们对荧光珠和生物样品进行了成像，使用结构照明成功地获得了对比度更高的光学切片图像，使用旋转照明成功地减少了阴影效应。(C)2016年度美国光学学会
We show that light sheet fluorescence microscopy with structured and pivoting illumination enables fast image acquisition and improved image quality. A one-dimensional spatial light phase modulator is used to control the illumination profile at high speed. To demonstrate the features of the system, we image fluorescent beads and biological samples, successfully obtaining optically sectioned images with higher contrast using structured illumination and with reduced shadowing effects using pivoting illumination. (C) 2016 Optical Society of America