A neuronal antigen in the brains of Alzheimer patients.
A neuronal antigen in the brains of Alzheimer patients.
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DOI：
10.1126/science.3083509
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发表时间：
1986-05
期刊：
影响因子：
56.9
通讯作者：
B. Wolozin;Alex Pruchnicki;D. Dickson;P. Davies
中科院分区：
文献类型：
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作者：
B. Wolozin;Alex Pruchnicki;D. Dickson;P. Davies
制备了一种针对阿尔茨海默病患者脑组织混合匀浆的单克隆抗体。这种抗体可以识别一种抗原，这种抗原在阿尔茨海默病患者大脑的某些区域比在正常大脑中浓度高得多。这种抗原似乎是一种蛋白质，存在于参与形成神经斑块和神经原纤维缠结的神经元中，也存在于阿尔茨海默病大脑切片中一些形态正常的神经元中。部分纯化和Western blot分析表明，来自阿尔茨海默病大脑的抗原是一个分子量为68,000的单一蛋白质。将相同的纯化程序应用于正常脑组织，可以检测到少量分子量较低的蛋白质。
A monoclonal antibody was prepared against pooled homogenates of brain tissue from patients with Alzheimer's disease. This antibody recognizes an antigen present in much higher concentration in certain brain regions of Alzheimer patients than in normal brain. The antigen appears to be a protein present in neurons involved in the formation of neuritic plaques and neurofibrillary tangles, and in some morphologically normal neurons in sections from Alzheimer brains. Partial purification and Western blot analysis revealed the antigen from Alzheimer brain to be a single protein with a molecular weight of 68,000. Application of the same purification procedure to normal brain tissue results in the detection of small amounts of a protein of lower molecular weight.