Procedures for cryogenic X-ray ptychographic imaging of biological samples.
Procedures for cryogenic X-ray ptychographic imaging of biological samples.
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DOI：
10.1107/s2052252516020029
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发表时间：
2017-03-01
期刊：
影响因子：
3.9
通讯作者：
Robinson IK
中科院分区：
文献类型：
--
作者：
Yusuf M;Zhang F;Chen B;Bhartiya A;Cunnea K;Wagner U;Cacho-Nerin F;Schwenke J;Robinson IK
描述了一种新的实验装置，用于在低温条件下使用钻石光源的光束线I13成像人类染色体。生物样品制备程序已开发成像人类染色体在低温条件下。描述了一种新的实验装置，用于在钻石光源下使用光束线I13成像冷冻样品。这篇手稿描述了设备和实验程序，以及作者的第一次胸电图重建使用x射线。
A new experimental setup, developed for imaging human chromosomes under cryogenic conditions using beamline I13 at Diamond Light Source, is described. Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors’ first ptychographic reconstructions using X-rays.