Renal interstitial fibrosis (RIF): II. Ultrasound follow up study of single uranyl nitrate administration causing renal dysfunction in rats - comparison with histologic and functional renal parameters
Renal interstitial fibrosis (RIF): II. Ultrasound follow up study of single uranyl nitrate administration causing renal dysfunction in rats - comparison with histologic and functional renal parameters
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DOI：
10.1078/0940-2993-00227
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发表时间：
2002-07-01
影响因子：
--
通讯作者：
Fleck, C
中科院分区：
文献类型：
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作者：
Fleck, M;Appenroth, D;Fleck, C
单次给予硝酸铀酰（UN; 0.5 mg/100 g.b.重量注射用）成年雌性Wistar大鼠可靠地诱导肾间质纤维化（RIF），提供了研究治疗策略的实验模型。本研究的目的是通过比较损伤后30周内的功能参数与超声检查，进一步验证我们之前研究过的UN诱导RIF大鼠模型（附录等，2001）。在UN给药后的急性期（第2 - 17天），可通过超声检测到炎症体征（肾血流量增加、肾皮质肿胀、肾盂增大）。四周后，UN导致功能变化（肌酐清除率降低，尿蛋白排泄增加和肾组织中OH-脯氨酸浓度增加）。在体外实验中，对氨基马尿酸的积累和胚胎发生减少。与肾功能改变相一致，超声检查显示，肾功能在术后10 ~ 30周明显改变，表现为肾脏大小、形态改变，肾灌注减少，肾盂增大。超声检查在小型实验室动物中的灵敏度有限，最适用于肾毒素给药后急性肾脏变化的随访研究。超声检查不能推荐用于慢性肾衰竭进展的无创筛查。
A single administration of uranyl nitrate (UN; 0.5 mg/100 g.b. wt. i.p.) to adult female Wistar rats reliably induces renal interstitial fibrosis (RIF) providing an experimental model to investigate therapeutic strategies. It was the aim of this study to further characterise a rat model of UN induced RIF which we have studied previously (APPENROTH et al. 2001) by the comparison of functional parameters with ultrasonographic examination over a period of 30 weeks after injury. In the acute phase after UN administration (between days 2 and 17) signs of inflammation (increase in renal blood flow, swelling of renal cortex, enlargement of renal pelvis) could be detected by ultrasound. After four weeks UN led to functional changes (decreased creatinine clearance, increased urinary protein excretion and increased OH-proline concentration in renal tissue). In vitro, the accumulation of paminohippurate and the gluconeogenesis were reduced. In accordance with the functional changes, distinct ultrasonographic abnormalities could be seen between weeks 10 and 30 after UN with regard to changes in kidney size and shape, reduced renal perfusion and enlargement of renal pelvis. The sensitivity of ultrasonography in small laboratory animals is limited and most useful for followup studies of acute renal changes after administration of nephrotoxins. Ultrasonography can not be recommended for non-invasive screening of the progression of chronic renal failure.