Glucose depletion causes haploid invasive growth in yeast
Glucose depletion causes haploid invasive growth in yeast
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DOI：
10.1073/pnas.240345197
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发表时间：
2000-12-05
影响因子：
11.1
通讯作者：
Sprague, GF
中科院分区：
文献类型：
--
作者：
Cullen, PJ;Sprague, GF
单倍体酵母侵入固体琼脂对营养限制的反应。为了破译入侵的线索，我们开发了一种单细胞侵入性生长测定法。使用这种测定，以及传统的平板洗涤测定，我们表明，发生侵入性生长响应于葡萄糖消耗。在不存在葡萄糖（或其它可发酵糖）的情况下，在第一次细胞分裂中，单个细胞采用非轴向出芽模式和伸长形态，并且在含有少至10个细胞的小菌落中观察到侵入琼脂。为了支持这一观察结果，我们发现葡萄糖抑制了STE 11 -4 pbs 2的高侵袭性生长形态。hs 17和RAS 2 V19突变。此外，从YPD培养基中去除葡萄糖引起野生型细胞的组成型侵袭。我们测试了葡萄糖控制蛋白在入侵中的作用，发现Snf 1是一种葡萄糖抑制基因去抑制所需的蛋白质，是入侵性生长所需的。转录因子Sip 4与Snf 1相互作用，并在二次移位过程中诱导，对侵袭性生长具有抑制作用，表明葡萄糖消耗依赖性侵袭需要多种机制。
Haploid yeast invades solid agar in response to nutrient limitation. To decipher the cues that underlie invasion, we have developed a single cell invasive growth assay. Using this assay, as well as the traditional plate-washing assay, we show that invasive growth occurs in response to glucose depletion. in the absence of glucose (or other fermentable sugar), individual cells adopted a nonaxial budding pattern and elongated morphology within the first cell divisions, and invasion into the agar was observed in microcolonies containing as few as 10 cells. In support of this observation, we found that glucose suppressed the hyperinvasive growth morphology of STE11-4 pbs2. hsl7, and RAS2V19 mutations. In addition, removal of glucose from YPD medium caused constitutive invasion in wild-type cells. We tested glucose control proteins for a role in invasion and found that Snf1, a protein required for derepression of glucose-repressed genes, was required for invasive growth. The transcription factor Sip4 which interacts with Snf1 and is induced during the diauxic shift, had an inhibitory role on invasive growth, suggesting that multiple mechanisms are required for glucose depletion-dependent invasion.