Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency.
Difference in expression patterns of placental cholesterol transporters, ABCA1 and SR-BI, in Meishan and Yorkshire pigs with different placental efficiency.
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不同胎盘效率梅山猪和约克夏猪胎盘胆固醇转运蛋白ABCA1和SR-BI表达模式的差异
DOI：
10.1038/srep20503
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发表时间：
2016-02-08
影响因子：
4.6
通讯作者：
Yu M
中科院分区：
文献类型：
--
作者：
Hong L;Xu X;Huang J;Lei M;Xu D;Zhao S;Yu M
胆固醇是一种关键的细胞膜成分和类固醇激素的前体。母体胆固醇是胚胎发育的重要外源性胆固醇来源，其转运由ABCA 1和SR-BI介导。在此，我们报道了在猪围着床期，ABCA 1在子宫腔上皮（LE）中表达，有趣的是，它在子宫系膜侧的LE中表达更丰富。然而，SR-BI主要由LE、腺上皮细胞（GE）和滋养层细胞（Tr）表达。在胎盘形成过程中，ABCA 1和SR-BI蛋白在梅山猪胎盘上皮细胞双层和胎盘乳晕的表达水平显著高于约克郡猪。梅山猪胎盘中胆固醇合成限速酶HMGCR的mRNA水平明显高于约克郡猪胎盘。我们的研究结果揭示了ABCA 1和SR-BI介导的猪胆固醇经胎盘转运的途径，并表明ABCA 1相关途径可能参与了妊娠锚定到子宫系膜侧。此外，梅山猪的胎盘效率对较小胎盘大小的反应可能是ABCA 1依赖性的代偿机制。
Cholesterol is a key cell membrane component and precursor of steroid hormones. The maternal cholesterol is an important exogenous cholesterol source for the developing embryos and its transportation is mediated by ABCA1 and SR-BI. Here we reported that during the peri-implantation period in pigs, ABCA1 was expressed by uterine luminal epithelium (LE) and interestingly, its expression was more abundantly in LE on mesometrial side of uterus. However, SR-BI was expressed primarily by LE, glandular epithelial cells (GE) and trophoblast cells (Tr). During the placentation period, the expression levels of ABCA1 and SR-BI proteins at epithelial bilayer and placental areolae were significantly higher in Chinese Meishan pigs compared to Yorkshire pigs. Consisitently, mRNA levels of HMGCR, the rate-limiting enzyme for cholesterol synthesis, were significantly higher in Meishan placentas than in Yorkshire placentas. Our findings revealed the routes of transplacental cholesterol transport mediated by ABCA1 and SR-BI in pigs and indicated that ABCA1 related pathway may participate in anchoring the conceptus to the mesometrial side of uterus. Additionally, an ABCA1 dependent compensatory mechanism related to the placental efficiency in response to the smaller placenta size in Meishan pigs was suggested.