MINERAL AND MATRIX ALTERATIONS IN THE BONES OF INCISORS-ABSENT (IA IA) OSTEOPETROTIC RATS
MINERAL AND MATRIX ALTERATIONS IN THE BONES OF INCISORS-ABSENT (IA IA) OSTEOPETROTIC RATS
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DOI：
10.1007/bf02554876
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发表时间：
1985-01-01
影响因子：
4.2
通讯作者：
MARKS, SC
中科院分区：
文献类型：
--
作者：
BOSKEY, AL;MARKS, SC
切牙缺失（ia/ia）骨硬化大鼠的骨在组织学和影像学表现以及力学性能方面与其正常同窝仔（ia/+）大鼠不同。本研究探讨了如何骨硬化干骺端和颅骨的矿物质和基质不同于正常对照组。11日龄和52日龄的骨硬化动物的骨骼具有比年龄匹配的对照组更高的灰分（矿物质）含量;骨硬化干骺端具有升高的氨基己糖含量，表明软骨的持久性。钙酸性磷脂磷酸盐复合物，参与在体内和体外的羟基磷灰石形成的启动，显着减少在所有的骨石化骨的含量。在破骨细胞活性有缺陷的骨硬化大鼠中，新矿物质的形成可能减少，并被现有晶体上矿物质的增加所取代。通过密度离心法对骨硬化骨颗粒进行分级，结果表明骨硬化骨比年龄匹配的对照组矿化程度更高。X-射线衍射分析的矿化馏分表明，缺乏矿物晶体的生长过程中的骨硬化动物的成熟。从高矿化的骨硬化骨部分的Ca：P比值升高和这些致密部分的高己糖胺含量来看，没有重塑是明显的。这些观察结果可以解释一些不寻常的机械性能的骨硬化骨。
The bones of incisors-absent (ia/ia) osteopetrotic rats differ from those of their normal littermates (ia/+) in histologic and radiographic appearance and in mechanical properties. This study examined how the mineral and matrices of osteopetrotic metaphyses and calvaria differed from normal controls. Bones of 11-day-old and 52-day-old osteopetrotic animals had higher ash (mineral) contents that age-matched controls; osteopetrotic metaphyses had elevated hexosamine contents, indicative of the persistence of cartilage. Ca acidic phospholipid phosphate complexes, involved in initiation of hydroyapatite formation in vivo and in vitro, were significantly reduced in content in all osteopetrotic bones. In the osteopetrotic rat, where osteoclast activity is defective, new mineral formation may be reduced and replaced by accretion of mineral on existing crystals. Fractionation of osteopetrotic bone particles by density centrifugation demonstrated that the osteopetrotic bone was much more mineralized than that of age-matched controls. X-ray diffraction analysis of the mineralized fractions indicated a lack of growth of mineral crystals during maturation of the osteopetrotic animals. The absence of remodeling was apparent from the elevated Ca:P ratios of the highly mineralized osteopetrotic bone fraction, and from the high hexosamine content of these dense fractions. These observations may explain some of the unusual mechanical properties of osteopetrotic bone.