Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
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DOI：
10.1063/1.5079306
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发表时间：
2019-01-01
期刊：
影响因子：
5.6
通讯作者：
Iida, Takuya
中科院分区：
文献类型：
--
作者：
Ueda, Mayu;Nishimura, Yushi;Iida, Takuya
纳米生物材料的自下而上的处理使得能够在自然系统中创建各种宏观结构。在这里，我们使用光学手段产生宏观组装结构的纳米粒子（NP）从蛋白质分子通过使用光诱导气泡（LIB）的产生下，不对称的压力驱动的流动在微通道中。组装纳米粒子的宽带光学响应有利于应用光子压力和光热对流时，通过使用红外激光照射。大量蛋白质的存在允许从光学组装的金属NP固定珠（MNFB）产生大量稳定的LIB。在更稀释的白蛋白溶液的情况下，单个LIB的收缩可以通过fg水平的白蛋白（在观察区域中为3.4 fg）引起MNFB的聚集，就像一个微型的泡泡糖。所得聚集体的大小可以通过改变蛋白质的浓度来控制。这些发现不仅可以用于设计宽带光学纳米复合材料的生产方法，还可以用于检测极少量蛋白质的无标记方法。(C)2019年作者。
Bottom-up processing of nanobiomaterials enables the creation of a variety of macroscopic structures in natural systems. Here, we use optical means to produce macroscopic-assembled structures of nanoparticles (NPs) from protein molecules by using light-induced bubble (LIB) generation under asymmetric pressure-driven flow in a microchannel. The broadband optical response of assembled NPs facilitates the application of photon pressure and photothermal convection when irradiated by using an infrared laser. The presence of a large amount of protein allows the generation of a vast number of stable LIBs from optically assembled metallic NP-fixed beads (MNFBs). In the case of more diluted albumin solutions, the shrinking of a single LIB can cause the aggregation of MNFBs via fg-level albumin (3.4 fg in the observation region), like a microscale bubblegum. The size of the resulting aggregate can be controlled by changing the concentration of protein. These findings can be used to devise production methods not only for broadband optical nanocomposites but also for label-free methods to detect an extremely small amount of protein. (C) 2019 Author(s).