Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus
Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus
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DOI：
10.1099/0022-1317-80-5-1323
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发表时间：
1999-05-01
影响因子：
3.8
通讯作者：
Maeda, S
中科院分区：
文献类型：
--
作者：
Gomi, S;Majima, K;Maeda, S
对家蚕（Bombyx mori, Bm）致病性核多角体病毒（NPV， T3株）的基因组进行了测序和分析。BmNPV基因组全长128413个核苷酸，G+C含量为40%，包含136个开放阅读框（orf），编码60多个氨基酸的预测蛋白。BmNPV和加利福尼亚多核衣壳NPV （AcMNPV）虽然在表型上不同，但在基因组组织上有着密切的相关性。BmNPV基因组与AcMNPV基因组的约四分之三相同，超过90%。预测BmNPV与AcMNPV对应orf的氨基酸序列相关性约为90%，但BmNPV基因组缺乏Ac3 （conotoxin）、Ac7 （orf603）、Ac48 （etm）、Ac49 （pcna）、Ac70 （hcf-1）、Ac86 （pnk/pnl）和Ac134 （p94）的同源物。此外，BmNPV含有5个与Ac2相关的orf。在BmNPV和AcMNPV编码序列中也发现了高频率的多个3bp插入。
The genome of the nucleopolyhedrovirus (NPV) (T3 strain) pathogenic for Bombyx mori (Bm) was sequenced and analysed. The BmNPV genome was 128413 nucleotides long with a G+C content of 40% and contained 136 open reading frames (ORFs) encoding predicted proteins of over 60 amino acids. Although phenotypically different, the genome organizations of BmNPV and Autographa californica multinucleocapsid NPV (AcMNPV) were closely related. The BmNPV genome was over 90% identical to about three-quarters of the genome of AcMNPV. The relatedness of predicted amino acid sequences of corresponding ORFs between BmNPV and AcMNPV was about 90%, However, the BmNPV genome lacked homologues of the following AcMNPV ORFs: Ac3 (conotoxin), Ac7 (orf603), Ac48 (etm), Ac49 (pcna), Ac70 (hcf-1), Ac86 (pnk/pnl) and Ac134 (p94). In addition, BmNPV contained five ORFs related to Ac2. A high frequency of multiple 3 bp insertions was also found within BmNPV and AcMNPV coding sequences.