A preliminary investigation on the growth requirement for monovalent cations, divalent cations and medium ionic strength of marine actinomycete Salinispora
A preliminary investigation on the growth requirement for monovalent cations, divalent cations and medium ionic strength of marine actinomycete Salinispora
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DOI：
10.1007/s00253-009-2424-7
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发表时间：
2010-05-01
影响因子：
5
通讯作者：
Lam, Kin Sing
中科院分区：
文献类型：
--
作者：
Tsueng, Ginger;Lam, Kin Sing
本文报道了沙硝藻（Salinispora）沙硝藻（S. arenicola）、热带藻（S. tropica）和太平洋藻（S. pacifica）对镁和钙的生长需求，其中太平洋藻对镁和钙的生长需求最为严格。观察了这些离子之间的相互作用，以支持盐孢子的生长。我们还证明，钠的绝对需求，以支持Salinispora生长尚未确定，因为所有三种Salinispora都可以使用钾或锂来代替钠，以支持最大的生长。虽然锂可以代替钠最大限度地支持盐孢菌的生长，但对沙nicola的毒性大于热带和太平洋沙菌，在189 mM处抑制沙nicola的生长，但对热带和太平洋沙菌的生长没有影响。采用氯化钠基和氯化锂基培养基，我们发现Salinispora对二价离子、镁和钙都有生长需求，对离子强度（8.29 ~ 15.2 mS/cm）也有生长需求。沙藻对离子强度的生长要求低于热带沙藻和太平洋沙藻。
In this paper, we report that three species of Salinispora, S. arenicola, S. tropica, and S. pacifica, require magnesium and calcium, for growth, with S. pacifica having the most stringent growth requirement for these ions. Interaction between these ions in supporting the growth of Salinispora was observed. We also demonstrated that the absolute requirement of sodium to support the growth of Salinispora has not been established as all three species of Salinispora can use either potassium or lithium to replace sodium to support maximum growth. While lithium can replace sodium to support maximum growth of Salinispora, it is more toxic to S. arenicola than S. tropica and S. pacifica, inhibiting the growth of S. arenicola at 189 mM but without effect on the growth of S. tropica and S. pacifica. Using both sodium chloride-based and lithium chloride-based media, we showed that Salinispora has a growth requirement for divalent ions, magnesium and calcium as well as growth requirement for ionic strength (8.29 to 15.2 mS/cm). S. arenicola has a lower growth requirement for ionic strength than S. tropica and S. pacifica.