Murine epidermal cell-derived thymocyte-activating factor resembles murine interleukin 1.
Murine epidermal cell-derived thymocyte-activating factor resembles murine interleukin 1.
复制标题
鼠表皮细胞衍生的胸腺细胞激活因子类似于鼠白细胞介素 1。
DOI：
10.4049/jimmunol.128.5.2147
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发表时间：
1982
影响因子：
4.4
通讯作者：
J. Oppenheim
中科院分区：
文献类型：
--
作者：
T. Luger;B. Stadler;B. M. Luger;B. Mathieson;M. Mage;J. Schmidt;J. Oppenheim
我们先前已经描述了表皮细胞衍生的胸腺细胞活化因子（ETAF），这是由鼠PAM 212角质形成细胞系产生的。ETAF的生物学活性和生化特性与巨噬细胞源性白细胞介素1（IL 1）相似。IL 1和ETAF都能促进胸腺细胞增殖，增强淋巴细胞产生白细胞介素2（IL 2），在pH 4至11和-70 ° C至60 ° C下稳定的多肽。在这项研究中，我们描述了一种定量微量测定，以获得标准化的评估ETAF的活性，这使我们能够进一步确定ETAF的特点及其与IL 1的关系。正如刺激的巨噬细胞产生更多的IL 1活性一样，脂多糖（LPS）或二氧化硅刺激也增加了Pam 212角质形成细胞产生的ETAF活性。通过机械破坏角质形成细胞的汇合单层和通过在G1/S间期用羟基脲阻断PAM 212细胞的增殖，也获得了增加的水平。这些观察结果与伴随的角质形成细胞活力的降低一起表明，延长细胞周期因子ETAF产生的G1期的“有害”刺激。ETAF与鼠IL 1一样，具有5.2的等电点。对PHA和IL 1有反应的PNA-胸腺细胞的相同亚群负责对ETAF的增强的增殖反应。此外，与IL 1的情况一样，PNA- Lyt-2-胸腺细胞对ETAF最敏感，而PNA+ Lyt-2+胸腺细胞则不敏感.最后，ETAF活性，像IL 1一样，似乎是成纤维细胞的促有丝分裂信号。尽管ETAF由不同的细胞类型产生，但这些观察结果继续支持ETAF可能与IL 1相同或密切相关的观点。
We have previously described an epidermal cell-derived thymocyte-activating factor (ETAF), which is produced by the murine PAM 212 keratinocyte cell line. ETAF appeared to be similar to macrophage-derived interleukin 1 (IL 1) in its biologic activities and biochemical characteristics. Both IL 1 and ETAF augment thymocyte proliferation, enhance lymphocyte production of interleukin 2 (IL 2), and are 15,000 m.w. polypeptides that are stable at pH 4 to 11 and from -70 degrees C to 60 degrees C. In this study we describe a quantitative microassay to obtain standardized assessment of ETAF activity, which enabled us to further define the characteristics of ETAF and its relationship to IL 1. Just as stimulated macrophages produce more IL 1 activity, Pam 212 keratinocyte production of ETAF activity was increased by stimulation with lipopolysaccharide (LPS) or silica. Increased levels were also obtained by mechanical disruption of confluent monolayers of keratinocytes and by blocking proliferation of the Pam 212 cells with hydroxyurea at the G1/S interphase. These observations in conjunction with a concomitant decrease in keratinocyte viability suggest that "injurious" stimuli that prolong the G1 phase of the cell cycle factor ETAF production. ETAF, like murine IL 1, has an isoelectric point of 5.2. The same subpopulations of PNA-thymocytes that respond to PHA and IL 1 are responsible for the enhanced proliferative response to ETAF. Furthermore, as in the case of IL 1, PNA- Lyt-2- thymocytes were most responsive to ETAF, but not PNA+ LYt-2+ thymocytes. Finally, ETAF activity, like IL 1, appears to be a mitogenic signal for fibroblasts. Although produced by different cell types, these observations continue to support the view that ETAF may be identical or closely related to IL 1.