DNA ploidy and proliferating cell nuclear antigen image analysis of peritoneal and pleural effusions. A possible diagnostic role.
DNA ploidy and proliferating cell nuclear antigen image analysis of peritoneal and pleural effusions. A possible diagnostic role.
复制标题
腹膜和胸腔积液的 DNA 倍体和增殖细胞核抗原图像分析。
DOI：
10.1159/000332677
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发表时间：
1997
期刊：
影响因子：
1.8
通讯作者：
Marrogi,AJ
中科院分区：
文献类型：
--
作者：
el-Habashi,AH;Freeman,SM;el-Morsi,B;el-Didi,M;Martin,A;Marrogi,AJ
目的探讨DNA和增殖细胞核抗原（PCNA）图像分析（IA）在提高常规细胞学（CC）诊断敏感性中的作用。研究设计通过对87例连续性胸腹腔积液的组织病理学和临床资料进行分析，评价CC和DNA IA结果的准确性。结果CC具有敏感性、特异性、特异性和特异性，阳性预测值（PPV）和阴性预测值（NPV）分别为65%、100%、100%和62%。49例出现非整倍体峰，其中47例为真阳性。38例二倍体中30例为真阴性。其敏感性、特异性、阳性预测值和阴性预测值分别为85%、94%、96%和80%。DNA倍体与PCNA增殖指数（PI）呈正相关（R=. 697)PCNA PI在良、恶性胸腔积液中的表达差异有显著性（P<0. 05）。001).结论PCNA检测DNA IA PI可作为细胞学诊断不明确的CC的辅助诊断工具。
ObjectiveTo determine the role of DNA and proliferating cell nuclear antigen (PCNA) image analysis (IA) in enhancing the diagnostic sensitivity of conventional cytology (CC).Study designThe histopathologic and clinical data on 87 consecutive pleural and peritoneal effusions were used to evaluate the accuracy of CC and DNA IA results.ResultsCC showed a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 65%, 100%, 100% and 62%, respectively. Aneuploidy peaks were seen in 49 cases; 47 of them were true positives. Thirty of 38 diploid cases were true negatives. The sensitivity, specificity, PPV and NPV were 85%, 94%, 96% and 80%, respectively. There were positive correlations between DNA ploidy profile and PCNA proliferative index (PI),(R=. 697) and significant differences in PCNA PI between malignant and benign effusions (P<. 001).ConclusionThe DNA IA PI by PCNA can be used as a complementary diagnostic tool with CC in cytologically inconclusive cases.