EFFECT OF ETHANOL ON FATTY ACID METABOLISM - STIMULATION OF HEPATIC FATTY ACID SYNTHESIS IN VITRO
EFFECT OF ETHANOL ON FATTY ACID METABOLISM - STIMULATION OF HEPATIC FATTY ACID SYNTHESIS IN VITRO
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DOI：
10.1172/jci104266
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发表时间：
1961-01-01
影响因子：
15.9
通讯作者：
SCHMID, R
中科院分区：
文献类型：
--
作者：
LIEBER, CS;SCHMID, R
用不同浓度的c14标记的和未标记的乙醇、乙酸、葡萄糖、果糖和山梨醇孵育大鼠肝脏切片，并测定同位素在脂肪酸和CO2中的掺入情况。肝片总脂肪酸含量用乙醇孵育显著高于用葡萄糖或醋酸孵育。此外，与醋酸、葡萄糖和果糖相比，乙醇和山梨醇刺激了乙酸酯- c14进入肝片脂肪酸。这种刺激作用被认为与乙醇和山梨醇氧化形成的过量还原二磷酸吡啶核苷酸（DPNH）有关。DPN/DPNH比值的降低导致醋酸- c14向脂肪酸合成的转变，同时减少了C14O2的形成。
Rat liver slices were incubated with various concentrations of c14-labeled and unlabeled ethanol, acetate, glucose, fructose and sorbitol, and the incorporation of the isotope into fatty acids and CO2 was determined. The total fatty acid content of liver slices was significantly higher when incubated with ethanol than with glucose or acetate. Moreover, the incorporation of acetate-C14 into fatty acids of liver slices was stimulated by ethanol and sorbitol, as compared to acetate, glucose and fructose. This stimulatory effect is believed to be related to excess reduced diphosphopyridine nucleotide (DPNH) formed on oxidation of ethanol and sorbitol. The reduced DPN/DPNH ratio results in a shift in incorporation of acetate-C14 towards fatty acid synthesis, with concomitant reduction of C14O2 formation.