RNA levels of human retrovirus receptors Pit1 and Pit2 do not correlate with infectibility by three retroviral vector pseudotypes
RNA levels of human retrovirus receptors Pit1 and Pit2 do not correlate with infectibility by three retroviral vector pseudotypes
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DOI：
10.1089/10430349850019454
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发表时间：
1998-11-20
影响因子：
4.2
通讯作者：
Pedersen, L
中科院分区：
文献类型：
--
作者：
Uckert, W;Willimsky, G;Pedersen, L
用北方分析和定量RT-PCR方法分别测定了不同受体Pit 1和Pit 2的mRNA水平。坑1。Pit 1和Pit 2在人体组织和细胞系中的表达量不同，Pit 1特异性mRNA的表达量普遍高于Pit 2 mRNA。Pit 1和Pit 2 RNA水平与GaLV和A-MuLV假型载体的感染性之间没有相关性。GaLV和A-MuLV表现出部分相互干扰。MuLV-10A 1可以利用浴Pit 1和Pit 2进入细胞，但不能比A-MuLV或GaLV更有效地感染14种人细胞系中的任何一种。干扰试验表明，MuLV-10A 1对大多数细胞具有较高的亲和力，并且主要通过Pit 2感染大多数细胞。然而，至少在一个细胞系中，它更有效地使用Pit 1进入。我们的结论是：（1）人细胞中Pit 1和Pit 2的mRNA水平并不能分别指示GaLV和A-MuLV假型的感染性;（2）A-MuLV可以像GaLV一样有效地感染靶细胞，尽管Pit 2 RNA的丰度低于Pit 1 RNA;（3）除了Pit 1和Pit 2的存在之外，其他因子也参与了逆转录病毒感染;和（4）MuLV-10A 1假型并不比A-MuLV和GaLV假型更有效地感染人细胞。
The gibbon ape leukemia virus (GaLV) and the amphotropic murine leukemia virus (A-MuLV) infect human cells via specific receptors, Pit1 and Pit2, respectively, mRNA levels of these receptors were determined by Northern analysis and for Pit2 in addition by quantitative RT-PCR. Pit1. and Pit2 were expressed in different amounts in human tissues and cell lines; Pit1-specific mRNA was generally more abundant than Pit2 mRNA. No correlation was found between Pit1 and Pit2 RNA levels and infectibility by GaLV and A-MuLV pseudotyped vectors, respectively. GaLV and A-MuLV revealed a partial reciprocal interference. MuLV-10A1 can utilize bath Pit1 and Pit2 for entry into cells but could not infect any of the 14 human cell Lines more efficiently than A-MuLV or GaLV. Interference assays suggested that MuLV-10A1 has a higher affinity for and infected most cells predominantly by Pit2. However, at least in one cell line it used Pit1 more efficiently for entry. We conclude that (1) Pit1 and Pit2 mRNA levels in human cells are not indicative of the infectibility by GaLV and A-MuLV pseudotypes, respectively; (2) A-MuLV can infect target cells as efficiently as can GaLV, although Pit2 RNA is less abundant than Pit1 RNA; (3) factor(s) in addition to the presence of Pit1 and Pit2 are involved in retroviral infection; and (4) MuLV-10A1 pseudotype does not infect human cells more efficiently than do A-MuLV and GaLV pseudotypes.