Influence of maternal, infant, and collection characteristics on high-quality cord blood units in Guangzhou Cord Blood Bank
Influence of maternal, infant, and collection characteristics on high-quality cord blood units in Guangzhou Cord Blood Bank
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DOI：
10.1111/trf.13126
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发表时间：
2015-09-01
期刊：
影响因子：
2.9
通讯作者：
Liao, Can
中科院分区：
文献类型：
--
作者：
Wu, Shaoqing;Xie, Guie;Liao, Can
背景：脐带血库的运作需要大量的人力、物力和财力。因此，提高储存的合格临床使用的高质量脐带血单位（HQCBU）的比例对于有效利用有限的资源至关重要。了解的因素，有助于HQCBUs，包括产妇，胎儿，和处理条件，可能会提高HQCBUs在storage.Study设计和方法的数量：4613 CBUs在广州脐带血库的产妇，胎儿，和处理条件进行了回顾性分析，以确定其对HQCBUs的影响。结果：胎龄大于37孕周、女性胎儿、脐带血量大于80 mL、出生体重大于3500 g、阴道分娩、脐带血采集和处理时间短（12 h）等因素可能导致HQCBUs。我们首次报道α-地中海贫血携带者表现出处理后总有核细胞计数（p-TNCC）增加至至少1.25 x 10（9），CD 34+细胞计数增加至至少6.01 x 10（6）。胎粪污染的羊水和25岁以下的母亲表现出p-TNCC增加到至少1.25 × 10（9），集落形成单位增加到至少23.24 × 10（5）。结论：我们确定了几个影响HQCBUs的因素。这些结果可作为更新CB收集策略的参考，优先考虑从大于37孕周的女性胎儿中收集CBU，高出生体重，以及从年龄小于25岁的母亲阴道分娩，特别是父母一方携带性状或羊水粪染的新生儿。收集的CBU应尽快送实验室进行优先处理，这将有助于提高HQCBU的数量和比例，有效利用CBB资源。
BACKGROUND: The operation of cord blood banks (CBBs) requires immense labor, material, and financial resources. Thus, increasing the ratio of high-quality cord blood units (HQCBUs) in storage that are qualified for clinical use is critical for the efficient use of limited resources. Understanding the factors that contribute to HQCBUs, including maternal, fetal, and processing conditions, may improve the number of HQCBUs in storage.STUDY DESIGN AND METHODS: The maternal, fetal, and processing conditions of 4613 CBUs at the Guangzhou Cord Blood Bank were analyzed retrospectively to determine their effect on HQCBUs. All CBUs were obtained following strict standard operation procedures.RESULTS: Several factors may contribute to HQCBUs: fetal age older than 37 gestational weeks, female fetus, large cord blood (CB) volume (>80 mL), high birthweight (>3500 g), vaginal delivery, and a shorter amount of time between CB collection and processing (12 hr). We report for the first time that alpha-thalassemia carriers exhibit a postprocessing total nucleated cell count (p-TNCC) increase to at least 1.25 x 10(9) and an increase of the CD34+ cell count to at least 6.01 x 10(6). Meconiumstained amniotic fluid and mothers younger than 25 years of age exhibited increased p-TNCC to at least 1.25 x 10(9), and colony-forming units increased to at least 23.24 x 10(5).CONCLUSIONS: We identified several factors that affect HQCBUs. These results may be used as a reference for updating CB collection strategies, with priority given to collecting CBUs from female fetuses older than 37 gestational weeks, at high birthweight, and born by vaginal delivery from mothers younger than 25 years of age, especially newborns with one parent carrying the trait or with meconium-stained amniotic fluid. The collected CBUs should be sent to the laboratory as soon as possible for priority processing, which will help to increase the number and ratio of HQCBUs and the effective use of CBB resources.