Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction
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DOI：
10.1186/1742-2094-4-6
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发表时间：
2007-01-25
影响因子：
9.3
通讯作者：
Cardinali, Daniel P.
中科院分区：
文献类型：
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作者：
Esquifino, Ana I.;Cano, Pilar;Cardinali, Daniel P.
雄性刘易斯大鼠（6周龄）接受相当于食物限制的33%或66%的热量限制。15天后，将7只动物的组注射完全弗氏佐剂加脊髓匀浆（SCH）以诱导实验性过敏性脑脊髓炎（EAE）或单独注射完全弗氏佐剂。EAE的定义仅基于临床。每天评估大鼠的EAE临床体征，并在免疫后第15天处死。饮食和SCH注射均显著降低体重。与接受正常饮食或33%热量限制饮食的大鼠相反，接受严重热量限制的大鼠未表现出EAE的临床体征。伴随着缺乏疾病的表现，66%的热量限制注射SCH大鼠脾和淋巴结伴刀豆球蛋白A（刀豆球蛋白A）的有丝分裂反应显着减少和较高的脾反应脂多糖。观察到脾脏、淋巴结和胸腺CD 4（+）细胞减少，脾脏和淋巴结CD 8（+）和CD 4（+）-CD 8+细胞数量增加，脾脏T、B和T-B细胞以及淋巴结和胸腺B和T-B细胞减少。有受损的干扰素（IFN）-γ的生产发生在三个检查组织。这些结果与EAE急性期可以通过严格的热量限制来减少的观点是一致的，这可能是通过IFN-γ的产生受损。
Male Lewis rats (6 weeks-old) were submitted to a calorie restriction equivalent to 33% or 66% of food restriction. Fifteen days later, groups of 7 animals were injected with complete Freund's adjuvant plus spinal cord homogenate (SCH) to induce experimental allergic encephalomyelitis (EAE) or with complete Freund's adjuvant alone. EAE was defined solely on clinical grounds. Rats were assessed daily for clinical signs of EAE and were killed on day 15 after immunization. Both diet and SCH injection diminished body weight significantly. In contrast to rats receiving a normal diet or a 33% calorie-restricted diet, rats subjected to severe calorie restriction did not exhibit clinical signs of EAE. Concomitantly with the lack of disease manifestation, 66% of calorie-restricted rats injected with SCH showed significantly less splenic and lymph node mitogenic response to concanavalin A (Con A) and a higher splenic response to lipopolysaccharide. Fewer splenic, lymph node and thymic CD4(+) cells, greater numbers of splenic and lymph node CD8(+) and CD4(+)- CD8+ cells, and fewer splenic T, B and T-B cells, and lymph node and thymic B and T-B cells were observed. There was impaired interferon (IFN)-gamma production occurred in the three examined tissues. The results are compatible with the view that the acute phase of EAE can be curtailed by severe calorie restriction, presumably through impaired IFN-gamma production.