Bacterial microbiota in small brown planthopper populations with different rice viruses
Bacterial microbiota in small brown planthopper populations with different rice viruses
复制标题
携带不同水稻病毒的小型褐飞虱种群中的细菌微生物群
DOI：
10.1002/jobm.201700004
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发表时间：
2017-07-01
影响因子：
3.1
通讯作者：
Zhou, Yijun
中科院分区：
文献类型：
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作者：
Li, Shuo;Zhou, Changwei;Zhou, Yijun
小褐飞虱(SBPH)是传播水稻条纹病毒(RSV)和水稻黑条矮缩病毒(RBSDV)的重要病毒载体。昆虫共生体在昆虫适合性中起着至关重要的作用，但它们在SBPH传播病毒中的作用尚不清楚。在这里，我们使用Illumina 16S rRNA基因MiSeq测序研究了非致病、RSV感染和RBSDV感染的SBPH种群中的内共生菌群落。不同样品共产生281,803个16S rRNA基因的有效序列。序列分析从门到属的不同分类水平上揭示了这些细菌群在不同SBPH种群中的百分比。极一致的细菌多样性和丰度表明，RSV或RBSDV感染并不影响SBPH中共生菌的组成和丰度。值得注意的是，沃尔巴克氏菌在所有种群中都是优势种。沃尔巴克氏菌和SBPH之间的共生关系可能是今后研究和利用于防治害虫SBPH的潜在途径。
The small brown planthopper (SBPH) is an important virus vector, transmitting Rice stripe virus (RSV), and Rice black-streaked dwarf virus (RBSDV). Insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by SBPH. Here, we investigated endosymbiont communities in non-viruliferous, RSV-infected, and RBSDV-infected SBPH populations using Illumina 16S rRNA gene MiSeq sequencing. In total, 281,803 effective sequences of the 16S rRNA gene were generated from different samples. Sequence analysis revealed the percentages of these bacterial groups in different SBPH populations on several taxonomic levels ranging from phyla to genera. The extremely consistent bacterial diversity and abundance indicated that RSV or RBSDV infection did not affect the composition and abundance of symbionts in SBPH. It was notable that Wolbachia was dominant in all populations. The symbiosis between Wolbachia and SBPH might be potentially studied and utilized to control pest SBPH in the future.