Infrared ion spectroscopy in a modified quadrupole ion trap mass spectrometer at the FELIX free electron laser laboratory
Infrared ion spectroscopy in a modified quadrupole ion trap mass spectrometer at the FELIX free electron laser laboratory
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DOI：
10.1063/1.4964703
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发表时间：
2016-10-01
影响因子：
1.6
通讯作者：
Oomens, Jos
中科院分区：
文献类型：
--
作者：
Martens, Jonathan;Berden, Giel;Oomens, Jos
我们报告的保罗型四极杆离子阱质谱仪的改进，并讨论其在红外离子光谱实验中的应用。主要的修改涉及光学访问的捕获离子和硬件和软件耦合到各种红外激光源在FELIX红外自由电子激光实验室。与先前描述的FELIX实验室的红外离子光谱实验相比，我们发现效率和灵敏度有了显着提高。探讨了离子俘获条件对红外多光子解离光谱的影响。在离子阱中，在较低的压力下发现增强的光解离。发现在减压条件下获得的光谱更接近地模仿在高真空条件下获得的傅里叶变换离子回旋共振质谱仪。本发明描述了一种气体混合系统，其能够将第二气体受控地添加到流入阱中的氦缓冲气体中，并且允许阱中的离子/分子反应。质谱仪的电子转移解离（ETD）选项允许对ETD生成的肽解离产物进行IR结构表征。出版社：AIP Publishing
We report on modifications made to a Paul-type quadrupole ion trap mass spectrometer and discuss its application in infrared ion spectroscopy experiments. Main modifications involve optical access to the trapped ions and hardware and software coupling to a variety of infrared laser sources at the FELIX infrared free electron laser laboratory. In comparison to previously described infrared ion spectroscopy experiments at the FELIX laboratory, we find significant improvements in efficiency and sensitivity. Effects of the trapping conditions of the ions on the IR multiple photon dissociation spectra are explored. Enhanced photo-dissociation is found at lower pressures in the ion trap. Spectra obtained under reduced pressure conditions are found to more closely mimic those obtained in the high-vacuum conditions of an Fourier transform ion cyclotron resonance mass spectrometer. A gas-mixing system is described enabling the controlled addition of a secondary gas into helium buffer gas flowing into the trap and allows for ion/molecule reactions in the trap. The electron transfer dissociation (ETD) option of the mass spectrometer allows for IR structure characterization of ETD-generated peptide dissociation products. Published by AIP Publishing.