Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming
Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming
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DOI：
10.1016/j.celrep.2019.10.082
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发表时间：
2019-11-19
期刊：
影响因子：
8.8
通讯作者：
Kuijpers, Taco W.
中科院分区：
文献类型：
--
作者：
Hoogendijk, Arie J.;Pourfarzad, Farzin;Kuijpers, Taco W.
人类中性粒细胞形成了最大的天然免疫细胞池，用于宿主防御细菌和真菌病原体。伴随着骨髓中增殖的髓系祖细胞向成熟的、不分裂的多形核血细胞转变的动态变化仍然不清楚。利用基于质谱学的定量蛋白质组学和转录转录数据，我们报道了骨髓和血液中五个发育阶段的动态变化。转录组和蛋白质组的整合揭示了人中性粒细胞发育过程中RNA和蛋白质动力学之间的高度动态和差异相互作用，这可能与典型的终末期血液中性粒细胞杀伤活动的功能成熟有关。
Human neutrophilic granulocytes form the largest pool of innate immune cells for host defense against bacterial and fungal pathogens. The dynamic changes that accompany the metamorphosis from a proliferating myeloid progenitor cell in the bone marrow into a mature non-dividing polymorphonuclear blood cell have remained poorly defined. Using mass spectrometry-based quantitative proteomics combined with transcriptomic data, we report on the dynamic changes of five developmental stages in the bone marrow and blood. Integration of transcriptomes and proteome unveils highly dynamic and differential interactions between RNA and protein kinetics during human neutrophil development, which can be linked to functional maturation of typical end-stage blood neutrophil killing activities.