CALCULATING THERMODYNAMIC DATA FOR TRANSITIONS OF ANY MOLECULARITY FROM EQUILIBRIUM MELTING CURVES
CALCULATING THERMODYNAMIC DATA FOR TRANSITIONS OF ANY MOLECULARITY FROM EQUILIBRIUM MELTING CURVES
复制标题
DOI：
10.1002/bip.360260911
复制
发表时间：
1987-09-01
期刊：
影响因子：
2.9
通讯作者：
BRESLAUER, KJ
中科院分区：
文献类型：
--
作者：
MARKY, LA;BRESLAUER, KJ
在本文中，我们推导出所需的方程的一般形式提取热力学数据的平衡过渡曲线上的任何分子的寡聚和多聚核酸。值得注意的是，由于这些方程和方案是通用的，因此它们也可用于表征核酸以外系统中的热力学平衡过程。我们简要地回顾了如何减少形式的一般方程已被许多研究人员用来评估单和双分子的过渡，然后解释如何将这些方程可以推广到计算热力学参数从常见的实验观测值的过渡更高的分子量。我们强调每种数据分析方法的优点和缺点，以便研究人员可以选择最适合他们的实验环境的方法。我们还描述了如何分析量热热容曲线和noncalorimetric微分熔化曲线，以提取模型无关和模型相关的热力学数据的过渡的任何分子。本文中描述的一般方程和分析方法应该是特别感兴趣的实验室，目前正在调查的关联和解离过程中的核酸表现出分子量大于2。
In this paper, we derive the general forms of the equations required to extract thermodynamic data from equilibrium transition curves on oligomeric and polymeric nucleic acids of any molecularity. Significantly, since the equations and protocols are general, they also can be used to characterize thermodynamically equilibrium processes in systems other than nucleic acids. We briefly review how the reduced forms of the general equations have been used by many investigators to evaluate mono- and bimolecular transitions, and then explain how these equations can be generalized to calculate thermodynamic parameters from common experimental observables for transitions of higher molecularities. We emphasize the strengths and weaknesses of each method of data analysis so that investigators can select the approach most appropriate for their experimental circumstances. We also describe how to analyze calorimetric heat capacity curves and noncalorimetric differentiated melting curves so as to extract both model-independent and model-dependent thermodynamic data for transitions of any molecularity. The general equations and methods of analysis described in this paper should be of particular interest to laboratories that currently are investigating association and dissociation processes in nucleic acids that exhibit molecularities greater than two.