Molecular identification and functional analysis of X-linked inhibitor of apoptosis-associated factor-1 (XAF1) in grass carp, Ctenopharyngodon idella
Molecular identification and functional analysis of X-linked inhibitor of apoptosis-associated factor-1 (XAF1) in grass carp, Ctenopharyngodon idella
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DOI：
10.1016/j.fsi.2023.108635
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发表时间：
2023-02-25
影响因子：
4.7
通讯作者：
Wei，Lili
中科院分区：
文献类型：
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作者：
Fu，Jianping;Chen，Jun;Wei，Lili
X连锁凋亡抑制蛋白（XIAP）相关因子1（XAF 1）是一个受干扰素刺激的基因，具有促凋亡作用。本研究对草鱼XAF 1基因进行了克隆和表达分析，并对其表达模式和功能进行了初步研究。XAF 1的开放阅读框（ORF）全长789个核苷酸，编码262个氨基酸。SMART在线检索结果显示，XAF 1基因中存在1个C2 H2型和6个C2 HC型锌指结构，而草鱼XAF 1基因与斑马鱼的同源性较高（71%），与四足动物的同源性较低（21-22%）。RT-qPCR结果显示XAF 1在所有检测的器官/组织中组成型表达，其中在血液中的表达最高。XAF 1在外周血白细胞（PBLs）和C. C. idellusrecombinant interferon-γ（rIFNg）.过表达XAF 1的CIK细胞对草鱼呼肠孤病毒（GCRV）具有抗性，对顺铂更敏感。这些结果暗示了XAF 1在进化中的功能同源物，但其机制可能需要进一步研究。
X-linked inhibitor of apoptosis protein (XIAP) -associated factor 1 (XAF1) is an interferon-stimulated gene which exhibits pro-apoptosis effect. In this study, XAF1 was characterized from grass carpCtenopharyngodon idellaand its expression pattern and function were analyzed. The open reading frame (orf) of XAF1 is 789 nucleotides (nt) encoding 262 amino acids. SMART online search results showed that a C2H2-type and six C2HC-type zinc-fingers were found in XAF1, however, the XAF1 of grass carp showed high sequence identity to zebrafish (71%), low sequence identity to tetrapods (21–22%). Rt-qPCR results showed that XAF1 was constitutively expressed in all tested organs/tissues with highest expression in blood. An inductive expression of XAF1 at mRNA level was observed in peripheral blood leucocytes (PBLs) andC. idelluskidney cells (CIKs) after treatment withC. idellusrecombinant interferon-γ (rIFNg). Overexpressing XAF1 in CIKs exhibited resistance against grass carp reovirus (GCRV) and more sensitivity to cisplatin. These results implied a functional homologue of XAF1 in evolution, however the mechanism may require further investigation.