A loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation
A loss-of-function allele of a TAC1-like gene (SlTAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation
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DOI：
10.1007/s10681-019-2418-1
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发表时间：
2019-04
期刊：
影响因子：
1.9
通讯作者：
M. González-Arcos;Maria Esther Noronha Fonseca;Daniel Basílio Zandonadi;L. Peres;A. Arruabarrena;D. S. Ferreira;Z. Kevei;F. Mohareb;A. Thompson;L. Boiteux
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文献类型：
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作者：
M. González-Arcos;Maria Esther Noronha Fonseca;Daniel Basílio Zandonadi;L. Peres;A. Arruabarrena;D. S. Ferreira;Z. Kevei;F. Mohareb;A. Thompson;L. Boiteux
在不同的番茄育种群体中研究了直立叶表型的遗传基础，其中包括源自番茄‘LT05’的番茄品种（具有直立叶表型和均匀成熟，基因型uu） ×S。 pimpinellifollium‘TO-937’（具有野生型叶表型和绿色果肩，基因型UU）。直立叶表型作为半显性性状遗传，并与基因SlGLK2(Solyc10g008160)的双等位基因共分离。该基因组位置与先前描述的称为直立叶（Erl）的半显性突变一致。对“LT05”、“TO-937”和其他三个不相关的种质（具有野生型Erl+等位基因）的基因组进行了重新测序，目的是鉴定候选基因。比较基因组分析，包括参考基因组“Heinz 1706”（Erl+等位基因），鉴定出 Solyc10g009320 基因中的直立叶特异性单核苷酸多态性（SNP）。该 SNP 导致 Erlallele 中的谷氨酰胺密码子（存在于所有野生型基因组中）变为 TAA（= ochre 终止密码子），从而产生了预测突变蛋白的较小版本（221 个氨基酸与 279 个氨基酸）。Solyc10g009320 之前被注释为“未知蛋白”，被鉴定为 TILLER ANGLE CONTROL1 样基因。通过对两个变异等位基因的 PCR 扩增子进行 Sanger 测序，证实了 ErlandSolyc10g009320 之间的关联。在 265 个 F2 个体中未检测到重组体。对比S7近等基因系对于每个替代等位基因也是纯合的，增强了Solyc10g009320作为强Erl候选基因的能力，并为育种计划中番茄结构的微调操作提供了可能性。
The genetic basis of an erectoid leaf phenotype was investigated in distinct tomato breeding populations, including one derived fromSolanum lycopersicum‘LT05’ (with the erectoid leaf phenotype and uniform ripening, genotypeuu) ×S. pimpinellifollium‘TO-937’ (with the wild-type leaf phenotype and green fruit shoulder, genotypeUU). The erectoid leaf phenotype was inherited as a semi-dominant trait and it co-segregated with theuallele of geneSlGLK2(Solyc10g008160). This genomic location coincides with a previously described semi-dominant mutation named asErectoid leaf(Erl). The genomes of ‘LT05’, ‘TO-937’, and three other unrelated accessions (with the wild-typeErl+allele) were resequenced with the aim of identifying candidate genes. Comparative genomic analyses, including the reference genome ‘Heinz 1706’ (Erl+allele), identified anErectoid leaf-specific single nucleotide polymorphism (SNP) in the geneSolyc10g009320. This SNP caused a change of a glutamine codon (present in all the wild-type genomes) to a TAA (= ochre stop-codon) in theErlallele, resulting in a smaller version of the predicted mutant protein (221 vs. 279 amino acids).Solyc10g009320, previously annotated as an ‘unknown protein’, was identified as aTILLER ANGLE CONTROL1-like gene. Linkage between theErlandSolyc10g009320was confirmed via Sanger sequencing of the PCR amplicons of the two variant alleles. No recombinants were detected in 265 F2individuals. Contrasting S7near-isogenic lines were also homozygous for each of the alternate alleles, reinforcingSolyc10g009320as a strongErlcandidate gene and opening the possibility for fine-tuning manipulation of tomato architecture in breeding programs.