High sugar diets can increase susceptibility to bacterial infection in Drosophila melanogaster
High sugar diets can increase susceptibility to bacterial infection in Drosophila melanogaster
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高糖饮食会增加果蝇细菌感染的易感性
DOI：
10.1101/2023.12.07.570705
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Darby A
中科院分区：
文献类型：
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作者：
Darby A
膳食糖营养过剩可通过通常未知的机制恶化包括昆虫和人类在内的多种生物的感染结果。在本研究中，我们发现喂食高糖饮食的成年黑腹果蝇更容易受到革兰氏阴性细菌普罗维登氏菌和粘质沙雷氏菌的感染。我们发现，在高糖饮食的环境下，黑胃菌群的增殖速度更快，导致宿主死亡的可能性增加。在高糖饮食环境下，黑胃菌群变得高血糖，我们发现额外的碳可用性可能会促进它们的生长。在寄主内生长。然而，我们没有发现证据表明碳供应的增加直接支持两种饮食中p . rettgergergrowth的增加。高糖饮食的黑胃显示AMP蛋白的产生减少。因此，膳食糖的营养过剩可能在AMP翻译水平上损害宿主免疫。我们的研究结果表明，膳食糖可以通过影响宿主和病原体来塑造感染动态，这取决于病原体的营养需求，并通过改变宿主维持免疫反应的生理能力。
Overnutrition with dietary sugar can worsen infection outcomes in diverse organisms including insects and humans, through generally unknown mechanisms. In the present study, we show that adultDrosophila melanogasterfed high-sugar diets became more susceptible to infection by the Gram-negative bacteriaProvidencia rettgeriandSerratia marcescens. We found thatP.rettgeriandS.marcescensproliferate more rapidly inD.melanogasterfed a high-sugar diet, resulting in increased probability of host death.D.melanogasterbecome hyperglycemic on the high-sugar diet, and we find evidence that the extra carbon availability may promoteS.marcescensgrowth within the host. However, we found no evidence that increased carbon availability directly supports greaterP.rettgerigrowth.D.melanogasteron both diets fully induce transcription of antimicrobial peptide (AMP) genes in response to infection, butD.melanogasterprovided with high-sugar diets show reduced production of AMP protein. Thus, overnutrition with dietary sugar may impair host immunity at the level of AMP translation. Our results demonstrate that dietary sugar can shape infection dynamics by impacting both host and pathogen, depending on the nutritional requirements of the pathogen and by altering the physiological capacity of the host to sustain an immune response.