Role of Zinc Sulphate in Immune Regulation in Artemisia annua Pollen-challenged P815 Mastocytoma Cells
Role of Zinc Sulphate in Immune Regulation in Artemisia annua Pollen-challenged P815 Mastocytoma Cells
复制标题
硫酸锌在青蒿花粉攻击的 P815 肥大细胞瘤细胞免疫调节中的作用
DOI：
10.1080/08820139.2019.1694939
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发表时间：
2020-01-08
影响因子：
2.8
通讯作者：
Yin，Shankai
中科院分区：
文献类型：
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作者：
Yang，Feng-Xia;Hou，Li;Yin，Shankai
摘要目的探讨硫酸锌对青蒿花粉攻击的P815肥大细胞瘤细胞的免疫调节作用。方法用不同浓度的硫酸锌和青蒿粉处理P815肥大细胞瘤细胞。用细胞计数试剂盒检测细胞增殖。用Western blotting检测细胞中ST2和p38的表达。采用酶联免疫吸附试验检测培养上清液中白介素33的水平。用流式细胞仪检测IL-2、IL-4、IL-5、干扰素-γ和肿瘤坏死因子的水平。结果0.001µg/mL青蒿粉可诱导P815肥大细胞瘤细胞产生过敏反应。IL-33、IL-4、ST2和p38的表达随着青蒿花粉浓度的升高而增加。50~200µmol/L的硫酸锌可促进P815肥大细胞瘤细胞的增殖。硫酸锌能抑制黄花蒿花粉诱导的IL-33、IL-4、ST2和p38的上调。结论硫酸锌能促进P815肥大细胞瘤细胞的增殖。还能减轻青蒿粉诱导的P815肥大细胞瘤细胞的变态反应，可能为变态反应性疾病的治疗提供新的方法。
ABSTRACT Objective This study aimed to investigate the role of zinc sulphate in immune regulation in Artemisia annua pollen-challenged P815 mastocytoma cells. Methods P815 mastocytoma cells were treated with various concentrations of zinc sulphate and Artemisia annua pollen. Cell proliferation was measured using the Cell Counting Kit-8. The amount of ST2 and p38 in the cells were measured using Western blotting. The level of interleukins (IL)-33 in the supernatant was determined using the enzyme-linked immunosorbent assay. The levels of IL-2, IL-4, IL-5, interferon-γ, and tumor necrosis factor were measured using the cytometric bead array. Results Artemisia annua pollen at a concentration >0.001 µg/mL induced allergic response in the P815 mastocytoma cells. Expressions of IL-33, IL-4, ST2, and p38 increased along with higher concentrations of Artemisia annua pollen. Zinc sulphate of 50–200 µmol/L promoted the proliferation of P815 mastocytoma cells. Zinc sulphate attenuated the upregulation of IL-33, IL-4, ST2, and p38 caused by Artemisia annua pollen. Conclusion Zinc sulphate can promote the proliferation of P815 mastocytoma cells. It can also attenuate allergic response in the P815 mastocytoma cells induced by Artemisia annua pollen, which might provide a new treatment method for allergic diseases.