Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network
Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network
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DOI：
10.1016/j.carbpol.2016.07.088
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发表时间：
2016-12-10
影响因子：
11.2
通讯作者：
Ciancia, Marina
中科院分区：
文献类型：
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作者：
Arata, Paula X.;Quintana, Irene;Ciancia, Marina
来自绿色海藻Cladophora falklandica的水溶性硫酸化木半乳阿拉伯聚糖由主要在C3上以及也在C2上部分硫酸化的4-连接的β-L-吡喃阿拉伯糖单元的主链构成。此外，在C2上还发现了以β-D-吡喃木糖残基、β-D-呋喃半乳糖残基或（1 -> 5）-和（1 -> 6）-键短链为主体的部分糖基化反应，这些化合物显示出抗凝活性，但活性远低于肝素。详细研究了纯化级分（F1）对纤维蛋白网络的影响。它改变了纤维蛋白形成的动力学，表明聚合过程受损。扫描电子显微镜显示一个宽松的构象，与较大的间隙孔比对照组。因此，该网络更容易裂解。这些纤维蛋白特性将减少凝块在血管中的持续时间，从而诱导较少的血栓形成状态。其抗凝作用的可能机制之一是直接抑制凝血酶。(C)2016爱思唯尔有限公司版权所有
The water-soluble sulfated xylogalactoarabinans from green seaweed Cladophora falklandica are constituted by a backbone of 4-linked beta-L-arabinopyranose units partially sulfated mainly on C3 and also on C2. Besides, partial glycosylation mostly on C2 with single stubs of p-D-xylopyranose, or single stubs of p-D-galactofuranose or short chains comprising (1 -> 5)- and/or (1 -> 6)-linkages, was also found. These compounds showed anticoagulant activity, although much lower than that of heparin. The effect of a purified fraction (F1) on the fibrin network was studied in detail. It modifies the kinetics of fibrin formation, suggesting an impaired polymerization process. Scanning electron microscopy showed a laxer conformation, with larger interstitial pores than the control. Accordingly, this network was lysed more easily. These fibrin properties would reduce the time of permanence of the clot in the blood vessel, inducing a lesser thrombogenic state. One of the possible mechanisms of its anticoagulant effect is direct thrombin inhibition. (C) 2016 Elsevier Ltd. All rights reserved.