DEVELOPMENT OF HEMATOPOIETIC STEM-CELL ACTIVITY IN THE MOUSE EMBRYO
DEVELOPMENT OF HEMATOPOIETIC STEM-CELL ACTIVITY IN THE MOUSE EMBRYO
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DOI：
10.1016/1074-7613(94)90081-7
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发表时间：
1994-07-01
期刊：
影响因子：
32.4
通讯作者：
DZIERZAK, E
中科院分区：
文献类型：
--
作者：
MULLER, AM;MEDVINSKY, A;DZIERZAK, E
在发育中的小鼠胚胎中第一次造血干细胞活动出现的确切时间是未知的。最近，小鼠胚胎发育中的腹-性腺-中肾区已被证明在受辐射小鼠中具有造血集落形成活性（CFU-S）。为了确定小鼠胚胎是否在类似的AGM区域具有确定的造血干细胞活性，并确定干细胞在卵黄囊、AGM区域和肝脏中出现的顺序，我们将这些胚胎组织转移到成年辐照受体中。我们在这里报告的长期，完整的，和功能性造血再生的主要和系列收件人与AGM衍生的细胞。我们观察到有效的造血干细胞活动的AGM区域之前出现的卵黄囊和肝干细胞的活动，并讨论了在小鼠胚胎发育的干细胞活动的成熟模型。
The precise time of appearance of the first hematopoietic stem cell activity in the developing mouse embryo is unknown. Recently the aorta-gonad-mesonephros region of the developing mouse embryo has been shown to possess hematopoietic colony-forming activity (CFU-S) in irradiated recipient mice. To determine whether the mouse embryo possesses definitive hematopoietic stem cell activity in the analogous AGM region and to determine the order of appearance of stem cells in the yolk sac, AGM region, and liver, we transferred these embryonic tissues into adult irradiated recipients. We report here the long-term, complete, and functional hematopoietic repopulation of primary and serial recipients with AGM-derived cells. We observe potent hematopoietic stem cell activity in the AGM region before the appearance of yolk sac and liver stem cell activity and discuss a model for the maturation of stem cell activity in mouse embryogenesis.