Testing the Influence of Habitat Structure and Geographic Distance on the Genetic Differentiation of Mouse Lemurs (Microcebus) in Madagascar
Testing the Influence of Habitat Structure and Geographic Distance on the Genetic Differentiation of Mouse Lemurs (Microcebus) in Madagascar
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测试栖息地结构和地理距离对马达加斯加鼠狐猴（Microcebus）遗传分化的影响
DOI：
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发表时间：
2015
影响因子：
2.5
通讯作者：
J. Ganzhorn
中科院分区：
文献类型：
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作者：
B. Scheel;J. Henke;Peggy Giertz;S. J. Rakotondranary;B. Hausdorf;J. Ganzhorn
马达加斯加生物区系中高度微地方病的进化仍然知之甚少。异地物种形成或沿连续梯度对当地条件的适应是导致狐猴非凡辐射的两种机制。在这里，我们测试了生活在马达加斯加西南部三个相邻但不同的植被层(从干燥森林到多刺灌木丛)的小狐猴(Microcebus Grisorufus)是否表现出遗传分化的迹象，这可以通过分子变异分析和基于距离的冗余分析来解释为早期物种形成和对不同环境条件的适应。在标记-重新捕获研究的背景下，2007年10月至2009年2月期间，在三个植被群的标准化诱捕网中设置了8次Sherman活体诱捕器，每次诱捕四个晚上，捕获老鼠狐猴。为了进行遗传分析，我们使用了41只雌性和35只雄性稻瘟病菌的组织样本。根据扩增片段长度多态(AFLP)数据(作为亲本标记)和线粒体D-loop序列(作为母系遗传标记)估计的DEME之间的遗传分化在雌性中比在雄性中更明显，这是该物种中分散的性别。在单因素分析中，按生态隔离与按距离隔离具有大致相同的统计效果，但一旦考虑按距离隔离，按生态隔离不再显著。尽管地方DEME之间存在一些差异，但根据遗传数据推断种群结构的贝叶斯方法STRUCTURAMA将所有个体分配到一个种群。因此，我们没有发现根据现有样本在小地理范围内适应三种调查的栖息地类型而导致早期生态物种形成的证据。
The evolution of the high level of microendemism in Madagascar’s biota remains poorly understood. Allopatric speciation or adaptations to local conditions along continuous gradients are two mechanisms that could have contributed to the extraordinary radiation of lemurs. Here we tested whether mouse lemurs (Microcebus griseorufus) occurring across three adjacent but distinct vegetation formations (ranging from dry forest to spiny bush) in southwestern Madagascar showed signs of genetic differentiation that could be interpreted as incipient speciation and adaptation to different environmental conditions using analysis of molecular variance and distance-based redundancy analysis. In the context of a mark-recapture study, mouse lemurs were captured with Sherman live traps set eight times for four nights per trapping session in standardized trapping grids in the three vegetation formations between October 2007 and February 2009. For genetic analyses, we used tissue samples from 41 female and 35 male M. griseorufus. Genetic differentiation between demes as estimated by amplified fragment length polymorphism (AFLP) data (as parental markers) and mitochondrial D-loop sequences (as maternally inherited markers) was more pronounced in philopatric females than in males, which are the dispersing sex in this species. In single-factor analyses, isolation-by-ecology had about the same statistical effect as isolation by distance, but isolation-by-ecology was no longer significant once isolation by distance was taken into account. Despite some differentiation between local demes, STRUCTURAMA, a Bayesian approach for inferring population structure from genetic data, assigned all individuals to a single population. Thus, we did not find evidence for incipient ecological speciation as a result of adaptation to the three investigated habitat types on a small geographic scale based on the available samples.
影响因子：
3.3
作者：
L. Excoffier;P. Smouse;J. Quattro
通讯作者：
L. Excoffier;P. Smouse;J. Quattro