The Translocation of 14C-photosynthate in Pisum sativum L.
The Translocation of 14C-photosynthate in Pisum sativum L.
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DOI：
10.1093/oxfordjournals.aob.a084747
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发表时间：
1973-09
期刊：
影响因子：
4.2
通讯作者：
D. Harvey
中科院分区：
文献类型：
--
作者：
D. Harvey
对生长在受控环境中的豌豆（Pisumsativum）和Orfac（Orfac）植株进行了短期的源库关系研究。14 C同化物的初级分配在24 h内完成，随后的24 h内没有明显的次级分配。荚果强烈但不完全依赖于对生叶。在叶片固定的14 C总量中，24 h内输出的比例与荚果的14 C库容量有关。就获得的每毫克cpm而言，后一种方法与适用于不溶性14 C-组织的湿燃烧法相当。
Short-term studies were undertaken of source and sink relationships inPisum sativum, cultivarOrfac, plants grown in a controlled environment.14C-distribution assays were conducted after 24 or 48 h14Cphotoassimilate translocation from a single leaf subtending either a vegetative or a reproductive node. The primary allocation of14C-assimilate was achieved within 24 h: there was no significant secondary movement of14C within the subsequent 24 h. The pod was strongly but not exclusively dependent on the subtending leaf. Out of the total14C fixed by a leaf the proportion that was exported within 24 h was related to the14C-sink capacity of the pods.A rapid non-combustive14C-assay method is described whereby14C-tissue fragments are rendered translucent prior to direct scintillation counting of the sample. In terms of cpm per mg obtained the latter method was comparable to a wet combustion method adapted for insoluble14C-tissue.