Microstructure and Ultrastructure Characteristics of Stigma and Style of Camellia oleifera
Microstructure and Ultrastructure Characteristics of Stigma and Style of Camellia oleifera
复制标题
DOI：
10.13275/j.cnki.lykxyj.2019.01.001
复制
发表时间：
2019-02-01
期刊：
影响因子：
--
通讯作者：
Yuan De-Yi
中科院分区：
文献类型：
--
作者：
Gao Chao;Yang Rui;Yuan De-Yi
[目的]从显微和超显微水平探讨油茶柱头和花柱的结构特征，为阐明油茶花柱自交不亲和反应的细胞学机制提供理论依据，同时为油茶杂交育种奠定理论基础。 [方法]以油茶开花当天的雌蕊为实验材料，采用细胞学方法，观察油茶柱头和花柱的显微和超显微结构特征，从细胞学角度进行研究。 [结果]油茶雌蕊由完整的柱头、花柱和子房组成。油茶有湿的柱头。乳头状细胞密集分布在接受面上。它们排列致密，细胞质浓缩，细胞器丰富。乳头细胞间有丰富的分泌物。一般组织细胞内含量较少，细胞器较少，细胞壁较薄，细胞壁外无分泌物。油茶的花柱由3～5个独立的花柱组成。花柱的上 2/3 彼此分离，而在花柱的下 2/3 和基部之间，它们开始融合。油茶的每种花柱都是中空的，由表皮、实质组织和花柱导管的内表面组成。管细胞外的区域充满大量分泌的粘液物质。细胞比实质组织中的细胞更有组织性和更大，具有致密的细胞质，并具有腺细胞的特征。风管从上到下逐渐变密，间隙尺寸不断减小。在花柱的底部，花柱导管相互融合，形成一个小空腔。 [结论]分析了油茶柱头和花柱的显微和超显微结构特征。柱头乳头和花柱导管的细胞是分泌细胞。花柱内实质组织细胞结构均匀。花柱导管从柱头到子房逐渐变窄。在样式的底部，每个样式的管道相互融合形成一个小空腔。其特点是花柱管细胞壁分泌的物质、细胞质密度大、细胞器种类数量有逐渐增加的趋势。
[Objective] To investigate the structural characteristics of the stigma and style of Camellia oleifera at the microscopic and ultramicroscopic levels and provide a theoretical basis for elucidating the cytological mechanism of breeding self-incompatibility reactions in C. oleifera style, while at the same time to establish a theoretical foundation for hybrid breeding of C. oleifera. [Method] Using the pistil of C. oleifera on the day of blooming as the experimental material, the microscopic and ultramicroscopic structural characteristics of C. oleifera stigma and styles were observed to study them from a cytological perspective using cytological method. [Result] The pistil of C. oleifera is composed of an intact stigma, style, and ovary. C. oleifera has a wet stigma. The papillary cells are densely distributed on the receptive surface. They are densely arranged with concentrated cytoplasm and abundant organelles. Abundant secretions are presented among papillary cells. Generally, there is less content and fewer organelles in tissue cells, the cell wall is thinner, and there are no secretions outside the cell wall. The style of C. oleifera is composed of 3 5 independent styles. The upper 2/3 of the style are separated from each other, whereas between the lower 2/3 and the base of style they begin to fuse. Each style of C. oleifera is hollow and composed of an epidermis, parenchymal tissue, and the inner surface of the style duct. The area outside the style duct cells is filled with large amounts of secreted mucous substances. The cells are more organized and larger than those in the parenchymal tissue, have dense cytoplasm, and have characteristics of glandular cells. The style duct becomes gradually denser from top to bottom with continuously decreasing gap size. At the base of the style, the style ducts fuse with each other, forming a small cavity. [Conclusion] The microscopic and ultramicroscopic structural characteristics of the stigma and style of C. oleifera is analyzed. The cells of the stigma papilla and style duct are secretory cells. The parenchymal tissue cells in the style have a uniform structure. The style duct gradually narrows from the stigma to the ovary. In the base of the style, each style duct fuses with each other to form a small cavity. It is characterized by the substances secreted by the style duct cell wall, cytoplasmic density, and a gradually increasing tendency in the number of types of organelles.