TRIMETHYLAMINE OXIDE RESPIRATION IN PROTEUS SP STRAIN NTHC153 - ELECTRON TRANSFER-DEPENDENT PHOSPHORYLATION AND L-SERINE TRANSPORT
TRIMETHYLAMINE OXIDE RESPIRATION IN PROTEUS SP STRAIN NTHC153 - ELECTRON TRANSFER-DEPENDENT PHOSPHORYLATION AND L-SERINE TRANSPORT
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DOI：
10.1128/jb.149.1.22-28.1982
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发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
STROM, AR
中科院分区：
文献类型：
--
作者：
STENBERG, E;STYRVOLD, OB;STROM, AR
用青霉素法将在葡萄糖和氧化三甲胺（TMAO）厌氧条件下生长的变形杆菌NTHC 153菌株的细胞转化为原生质球。通过渗透压休克裂解原生质球，并通过蔗糖梯度离心纯化膜囊泡。囊泡通电的电子转移甲酸TMAO显示积极的丝氨酸厌氧运输。变形杆菌属的厌氧无细胞提取物在法国压力池中破碎，用甲酸盐和NADH减少TMAO，同时形成有机磷酸盐。测定的净P/2 e-比值分别为0.1和0.3。NADH和TMAO依赖的磷酸化是敏感的氧化磷酸化解偶联剂（质子载体），甲酸和TMAO依赖的丝氨酸转运活动是敏感的离子载体和质子载体。变形杆菌TMAO的还原明显符合无氧呼吸的基本特征。
Cells of Proteus sp. strain NTHC153 grown anaerobically with glucose and trimethylamine oxide (TMAO) were converted to spheroplasts by the penicillin method. The spheroplasts were lysed by osmotic shock, and the membrane vesicles were purified by sucrose gradient centrifugation. Vesicles energized by electron transfer from formate to TMAO displayed active anaerobic transport of serine. An anaerobic cell-free extract of Proteus sp. disrupted in a French pressure cell reduced TMAO with formate and NADH with the concomitant formation of organic phosphate. The net P/2e- ratios determined were 0.1 and 0.3, respectively. The NADH- and TMAO-dependent phosphorylation was sensitive to uncouplers of oxidative phosphorylation (protonophores), and formate- and TMAO-dependent serine transport activities were sensitive to ionophores and protonophores. TMAO reduction in Proteus sp. evidently fulfills the essential features of anaerobic respiration.