A potential role for cadmium in the etiology of varicocele-associated infertility
A potential role for cadmium in the etiology of varicocele-associated infertility
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DOI：
10.1016/s0015-0282(97)81921-8
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发表时间：
1997-02-01
影响因子：
6.7
通讯作者：
Hershlag, A
中科院分区：
文献类型：
--
作者：
Benoff, S;Hurley, IR;Hershlag, A
目的：探讨精索静脉曲张患者精子中甘露糖配体受体和顶体反应缺陷是否与精液中过渡金属含量有关。设计：对有生育能力的男性、无精索静脉曲张且需要卵浆内单精子注射以实现受精的男性和有潜在精索静脉曲张相关性不育症的男性进行精液和血浆中镉和锌的检测。在外源镉存在和不存在的情况下，测定了可育供体精子肌动蛋白细胞骨架分布与顶体状态的关系。环境：大学医院分子生物学研究实验室。患者：来自两所大学医院的体外受精辅助生殖技术项目和两家男性泌尿科私人诊所的患者。干预措施：可育的供体精子在使能培养期间暴露于外源镉中，然后在高达41摄氏度的温度下培养。主要结果测量：用石墨炉原子吸收光谱法测定精液和血浆中的金属离子水平。检测运动精子的甘露糖配体结合以及自发和诱导顶体反应的能力。用抗肌动蛋白（antiactin）和抗肌动蛋白（antiyosin）抗体探测未固定的triton -通透精子。精索静脉曲张患者精浆中镉含量升高，锌含量降低。这些金属在精液和血液中的含量没有相关性。镉暴露在体外降低甘露糖受体表达、顶体胞吐和可育供体精子的细胞骨架形成。结论：过渡金属调节缺陷或过量镉暴露与精索静脉曲张相关性不孕症有关。
Objective: To determine whether mannose ligand receptor and acrosome reaction deficits in sperm from men with varicocele are related to the transition metal content of their semen.Design: Cadmium and zinc in semen and blood plasma were assayed for fertile males, men without varicocele who required intracytoplasmic sperm injection to achieve fertilization, and men evaluated for potential varicocele-associated infertility. The relationship between actin cytoskeletal distributions and acrosome status was determined for fertile donor sperm in the presence and absence of exogenous cadmium.Setting: University hospital-based molecular biology research laboratory.Patient(s): Patients from two university hospital-based IVF-assisted reproductive technology programs and two male urology private practices.Intervention(s): Fertile donor sperm were exposed to exogenous cadmium during capacitating incubations followed by culture at temperatures up to 41 degrees C.Main Outcome Measure(s): Metal ion levels in semen and blood plasma were determined by graphite furnace atomic absorption spectroscopy. Motile sperm were examined for mannose ligand binding and the ability to undergo spontaneous and induced acrosome reactions. Unfixed, Triton-permeabilized sperm were probed with antiactin and antimyosin antibodies.Result(s); Cadmium was elevated and zinc was decreased in the seminal plasma of men with varicocele. The content of these metals in semen and blood was not correlated. Cadmium exposure in vitro reduced mannose receptor expression, acrosome exocytosis, and cytoskeletal formation by fertile donor sperm.Conclusion(s): Defects in transition metal regulation or excessive cadmium exposure are involved in varicocele-associated infertility.